A new epithelial cell line, SSK from kidney of striped snakehead Channa striatus (Bloch 1793)


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Authors

  • D. K. Chaudhary ICAR-National Bureau of Fish Genetic Resources, Canal Ring Road, P. O. Dilkusha, Lucknow - 226 002 Uttar Pradesh, India
  • N. Sood ICAR-National Bureau of Fish Genetic Resources, Canal Ring Road, P. O. Dilkusha, Lucknow - 226 002 Uttar Pradesh, India
  • D. K. Verma ICAR-National Bureau of Fish Genetic Resources, Canal Ring Road, P. O. Dilkusha, Lucknow - 226 002 Uttar Pradesh, India
  • T. R. Swaminathan Peninsular and Marine Fish Genetic Resources Centre, ICAR-National Bureau of Fish Genetic Resources, CMFRI Campus, PB No. 1603, Kochi - 682 018, Kerala, India
  • B. Kushwaha ICAR-National Bureau of Fish Genetic Resources, Canal Ring Road, P. O. Dilkusha, Lucknow - 226 002 Uttar Pradesh, India
  • R. Abidi ICAR-National Bureau of Fish Genetic Resources, Canal Ring Road, P. O. Dilkusha, Lucknow - 226 002 Uttar Pradesh, India
  • P. K. Pradhan ICAR-National Bureau of Fish Genetic Resources, Canal Ring Road, P. O. Dilkusha, Lucknow - 226 002 Uttar Pradesh, India

https://doi.org/10.21077/ijf.2017.64.special-issue.76212-14

Abstract

A cell line was established from striped snakehead Channa striatus kidney. The cell line, named as SSK, has been passaged for over 62 times. Growth studies at different temperatures and foetal bovine serum (FBS) concentrations revealed that SSK cells show optimum growth at 28°C in L-15 medium containing 20% FBS. The chromosome number of SSK cells was 2n=40. Partial sequencing of mitochondrial cytochrome oxidase 1 gene of the cells confirmed that the cell line originated from C. striatus. The SSK cells were primarily epithelial, as determined using immunophenotyping. The cells from SSK cell line were transfected with phrGFP II-N mammalian expression vector. The SSK cell line was stored in liquid nitrogen (-196οC) at different passages and was successfully revived after four months of storage. The cell line could be successfully
employed for cytotoxicity studies, as revealed by neutral red assay. The cell line can be a valuable surrogate to the whole fish studies.

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Submitted

2017-12-01

Published

2018-03-14

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Articles

How to Cite

A new epithelial cell line, SSK from kidney of striped snakehead Channa striatus (Bloch 1793). (2018). Indian Journal of Fisheries, 64. https://doi.org/10.21077/ijf.2017.64.special-issue.76212-14