Development and validation of microsatellite markers in a protandrous fish species Eleutheronema tetradactylum (Shaw, 1804) through cross-species amplification


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Authors

  • Shihab Ismail Central Marine Fisheries Research Institute
  • N. Vineesh Central Marine Fisheries Research Institute Marine Biotechnology Division Research Scholar
  • C. Mohitha Central Marine Fisheries Research Institute Marine Biotechnology Division Research Scholar
  • P. Vijayagopal Central Marine Fisheries Research Institute Marine Biotechnology Division
  • A. Gopalakrishnan Central Marine Fisheries Research Institute

Abstract

The four fingered threadfin Eleutheronema tetradactylum (Shaw, 1804) is a prioritised species for mariculture in India. Their demand in the domestic markets is rapidly growing. Genetic stock structure analysis of fish populations is an important aspect from fisheries management perspective. The present study was conducted to develop microsatellite primers through cross-priming to elucidate the genetic structure of E. tetradactylum. A total of 13 polymorphic microsatellite markers were developed from the resource species, Pacific salmon Polydactylus sexfilis. The observed mean and the effective number of alleles were found to be 11.962 and 6.927 respectively. The mean of observed heterozygosity (Ho) and expected heterozygosity (He) values obtained were 0.784 and 0.798 respectively. These new microsatellite markers can be used as effective tools for studying genetic disparity as well as for elucidating evolutionary relationships among E. tetradactylum populations.

Author Biographies

  • Shihab Ismail, Central Marine Fisheries Research Institute

    Marine Biotechnology Division

    Research Scholar

  • N. Vineesh, Central Marine Fisheries Research Institute Marine Biotechnology Division Research Scholar

    Central Marine Fisheries Research Institute

    Marine Biotechnology Division

    Research Scholar

  • C. Mohitha, Central Marine Fisheries Research Institute Marine Biotechnology Division Research Scholar

    Central Marine Fisheries Research Institute

    Marine Biotechnology Division

    Research Scholar

  • P. Vijayagopal, Central Marine Fisheries Research Institute Marine Biotechnology Division

    Central Marine Fisheries Research Institute

    Marine Biotechnology Division

    Principal Scientist

     

  • A. Gopalakrishnan, Central Marine Fisheries Research Institute

    Central Marine Fisheries Research Institute

    Director

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Submitted

2018-07-23

Published

2018-09-30

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Section

Articles

How to Cite

Ismail, S., Vineesh, N., Mohitha, C., Vijayagopal, P., & Gopalakrishnan, A. (2018). Development and validation of microsatellite markers in a protandrous fish species Eleutheronema tetradactylum (Shaw, 1804) through cross-species amplification. Indian Journal of Fisheries, 65(3). https://epubs.icar.org.in/index.php/IJF/article/view/81674