Effect of salinity acclimatisation and administration of hormones on oocyte maturation, ovulation and spawning of goldspot mullet Liza parsia (Hamilton, 1822) reared in brackishwater pond


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Authors

  • Prem Kumar Fish culture division ICAR-Central Institute of Brackishwater Aquaculture, Kakdwip Research Centre West Bengal
  • G. Biswas Fish Culture Division, Kakdwip Research Centre of ICAR-CIBA
  • T.K. Ghoshal Nutritioin division, Kakdwip Research Centre of ICAR-CIBA
  • L. Christina Kakdwip Research Centre of CIBA.
  • P. Behera Kakdwip Research Centre of CIBA
  • M. Kailasam ICAR-CIBA

https://doi.org/10.21077/ijf.2019.67.1.90246-08

Abstract

Two separate experiments were conducted to evaluate the effect of salinity acclimatisation and administration of hormones on oocyte growth of Liza parsia (Hamilton, 1822). In the first experiment, broodstock fishes in the sex ratio of 2:1 (male:female) were acclimatised to  30 ppt salinity (which is the desired salinity for breeding L. parsia) in 24, 48 and 72 h of duration (n=6). Oocyte diameter from the experimental fishes were measured before and after salinity acclimatisation. The results showed that the acclimatisation to breeding salinity reduced oocyte diameter (p<0.05) in female fish and increased sperm relative mortality (p<0.05) in male fish. In the second experiment, males and females in the similar sex ratio (2:1) were acclimatised to the desired salinity of 30 ppt in 24 h. Subsequently, carp pituitary gland extract (PGE), human chorionic gonadotropin (HCG), combination of PGE+HCG and luteinising hormone releasing hormone analogue (LHRHa) with dopamine antagonist (DA) metoclopramide  were injected intramuscularly, The dosages used were: PGE-  0.01 and 0.02 mg g-1; HCG - 20 and 40 IU g-1, LHRHa  - 10 and 20 µg kg-1 as primary and secondary doses, respectively.  Metoclopramide was injected at the dose of 15 mg kg-1 along with LHRHa. Results of the second experiment indicated that the administration of PGE or PGE+HCG is essential for final oocyte maturation and ovulation in salinity acclimatised L. parsia.

Keywords:  Dopamine antagonist, Gonadotropin, Liza parsia, Luteinizing hormone, Oocytes growth, Salinity

Author Biographies

  • Prem Kumar, Fish culture division ICAR-Central Institute of Brackishwater Aquaculture, Kakdwip Research Centre West Bengal
    Fish culture Division, scientist
  • G. Biswas, Fish Culture Division, Kakdwip Research Centre of ICAR-CIBA
    Scientist,
  • T.K. Ghoshal, Nutritioin division, Kakdwip Research Centre of ICAR-CIBA
    Principal Scientist and SIC Kakdwip Research Cnetre of CIBA
  • L. Christina, Kakdwip Research Centre of CIBA.
    Scientist, Crustacean Culture Division
  • P. Behera, Kakdwip Research Centre of CIBA
    JRF, DBT project
  • M. Kailasam, ICAR-CIBA
    Principal Scientist and SIC, Fish Culture Division

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Submitted

2019-05-30

Published

2020-03-31

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Articles

How to Cite

Kumar, P., Biswas, G., Ghoshal, T., Christina, L., Behera, P., & Kailasam, M. (2020). Effect of salinity acclimatisation and administration of hormones on oocyte maturation, ovulation and spawning of goldspot mullet Liza parsia (Hamilton, 1822) reared in brackishwater pond. Indian Journal of Fisheries, 67(1). https://doi.org/10.21077/ijf.2019.67.1.90246-08