Prevalence and molecular characterisation of extended-spectrum β-lactamases genes in Escherichia coli and Salmonella spp. isolated from fish samples in Chhattisgarh, India


Abstract views: 345 / PDF downloads: 158

Authors

  • Rizwan Khan
  • Sanjay Shakya Professor and Head, Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences and A.H., CGKV, Anjora, Durg Chhattisgarh - 491001
  • Nitin Eknath Gade
  • Asit Jain
  • Anil Patyal Assistant Professor, Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences and A.H., CGKV, Anjora, Durg Chhattisgarh - 491001
  • S.L. Ali
  • Choodamani Chandrakar
  • Deeksha Deepak Hattimare
  • Ajeet Kumar Pandey

https://doi.org/10.21077/ijf.2021.68.2.93745-13

Abstract

coli and Salmonella spp. Out of 200 samples collected from retail markets of Durg, Raipur, Rajnandgaon and Balod
districts of Chhattisgarh State, 76 (38) and 46 (23%) samples were found contaminated with E. coli and Salmonella spp.,
respectively. Multiple antibiotic resistant (MAR) index varied between 0.00 to 0.88 and 0.11 to 0.88 for E. coli and Salmonella
isolates, respectively. The isolates were screened phenotypically for ESBL production and multiplex PCR targeting blaTEM,
blaSHV, andblaCTX-M genes, was carried out followed by sequencing. Prevalence of blaTEM, blaSHV and blaCTX-M genes in E.
coli and Salmonella isolates were found to be 13.2, 1.3, 0 and 6.5, 0, 4.4%, respectively. Higher percentage of resistant
genes was detected in plasmid DNA in comparison to genomic DNA. Sequencing of PCR amplicons of blaTEM and blaCTX-M
genes revealed the presence of blaTEM-116 and blaCTX-M-15 variants, respectively. The study indicates the presence of multidrug
resistant ESBL producing E. coli and Salmonella in fish samples, which may pose threat to the fish consumers.
Keywords: blaTEM, blaSHV, blaCTX-M, E. coli, Fish, MAR index, Salmonella

Downloads

Download data is not yet available.

Author Biography

Sanjay Shakya, Professor and Head, Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences and A.H., CGKV, Anjora, Durg Chhattisgarh - 491001

Professor and Head,

Department of Veterinary Public Health and Epidemiology

College of Veterinary Sciences and A.H.,

CGKV, Anjora, Durg, Chhattisgarh - 491001

Downloads

Submitted

2019-09-19

Published

2021-08-20

How to Cite

Khan, R., Shakya, S., Gade, N. E., Jain, A., Patyal, A., Ali, S., Chandrakar, C., Hattimare, D. D., & Pandey, A. K. (2021). Prevalence and molecular characterisation of extended-spectrum β-lactamases genes in Escherichia coli and Salmonella spp. isolated from fish samples in Chhattisgarh, India. Indian Journal of Fisheries, 68(2). https://doi.org/10.21077/ijf.2021.68.2.93745-13

Issue

Vol. 68 No. 2 (2021)

Section

Articles

License

The copyright of the articles published in Indian Journal of Fisheries vests with the Indian Council of Agricultural Research, who has the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in these journals. The Council has no objection in using the material, provided the information is being utilized for academic purpose but not for commercial use. Due credit line should be given to the ICAR where information will be utilized.
Citation