An improved in vitro germination medium for recalcitrant bread wheat (Triticum aestivum L.) pollen


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Authors

  • P. Jayaprakash ICAR-IARI, Regional Station, Wellington, The Nilgiris 643 231, Tamil Nadu
  • S. Annapoorani ICAR-IARI, Regional Station, Wellington, The Nilgiris 643 231, Tamil Nadu
  • V. K. Vikas ICAR-IARI, Regional Station, Wellington, The Nilgiris 643 231, Tamil Nadu
  • M. Sivasamy ICAR-IARI, Regional Station, Wellington, The Nilgiris 643 231, Tamil Nadu
  • J. Kumar ICAR-IARI Regional Station, Shimla, Himachal Pradesh
  • K. Saravannan Department of Genetics and Plant Breeding, Faculty of Agriculture, Annamalai University, Tamil Nadu
  • E. Punniakotti Department of Genetics and Plant Breeding, Faculty of Agriculture, Annamalai University, Tamil Nadu
  • D. Sheeba Department of Genetics and Plant Breeding, Faculty of Agriculture, Annamalai University, Tamil Nadu

Abstract

There is no robust and quick technique available for testing germination of wheat pollen. In this study, Brewbaker and Kwack (BK) pollen germination medium (PGM) was adjusted with á-amino caproic acid (EACA), peptone water and maltose to develop an improved medium for wheat. Germination of pollen in this PGM ranged from 92% to 98.2% depending upon genotype pollen germination media (PGM). This is the first report of PGM which has >95% wheat pollen germination in vitro.

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Submitted

2016-11-26

Published

2016-11-29

Issue

Vol. 75 No. 4 (2015): The Indian Journal of Genetics and Plant Breeding

Section

Articles

How to Cite

Jayaprakash, P., Annapoorani, S., Vikas, V. K., Sivasamy, M., Kumar, J., Saravannan, K., Punniakotti, E., & Sheeba, D. (2016). An improved in vitro germination medium for recalcitrant bread wheat (Triticum aestivum L.) pollen. The Indian Journal of Genetics and Plant Breeding, 75(4). https://epubs.icar.org.in/index.php/IJGPB/article/view/63204