Morpho-biochemical Characterization and Multivariate Assessment of Genetic Diversity in Rajmah (Phaseolus vulgaris L.) Genotypes from Northeast India

Genetic Diversity in Rajmah Genotypes


18 / 9

Authors

  • Bedashruti Saikia Assam Agricultural University, Jorhat, Assam
  • Rupam Borgohain Assam Agricultural University, Jorhat, Assam
  • Dr. Gayatree Goswami Assam Agricultural University, Jorhat, Assam
  • Ms. Jutika Das Assam Agricultural University, Jorhat, Assam
  • Mr. Anubrat Borah Assam Agricultural University, Jorhat, Assam

https://doi.org/10.56093/ijpgr.v39i2.174765

Keywords:

Biochemical traits, Genetic diversity, Multivariate analysis, Northeast India, Phaseolus vulgaris

Abstract

The present study assessed morphological, yield, and biochemical diversity among forty rajmah (Phaseolus vulgaris L.) genotypes
collected from five north eastern states of India, representing both vine and dwarf growth habits. Field experiments were conducted
during the rabiseasons of 2022–23 and 2023–24 at Assam Agricultural University, Jorhat, using a randomized block design with three
replications. Eighteen traits were studied, including ten morphological and eight biochemical parameters and were evaluated
through univariate and multivariate analyses. Analysis of variance revealed highly significant genotypic differences for all traits,
indicating substantial genetic variability. Vine types exhibited greater plant height, branching, pod number, and seed yield, whereas
dwarf types recorded higher 100-seed weight and superior nutritional attributes, including fat, fibre, niacin, vitamin B₆, and folic acid.
Correlation analysis showed strong positive associations between yield and key plant architecture traits. Principal component, cluster,
and heatmap analyses grouped genotypes primarily by growth habit and regional origin. The outcomes of the study signify rich
genetic diversity in the rajmah germplasm of Northeast India and identify key traits for simultaneous improvement of yield and
nutritional quality.

Downloads

Download data is not yet available.

Author Biographies

  • Bedashruti Saikia, Assam Agricultural University, Jorhat, Assam

    Department of Plant Breeding and Genetics, AAU, Jorhat

  • Rupam Borgohain, Assam Agricultural University, Jorhat, Assam

    Principal Scientist, Directorate of Research(Agri), AAU

  • Dr. Gayatree Goswami, Assam Agricultural University, Jorhat, Assam

    Project Scientist, Directorate of Research(Agri), AAU, Jorhat

  • Ms. Jutika Das, Assam Agricultural University, Jorhat, Assam

    Assistant Project Scientist, Directorate of Research(Agri), AAU, Jorhat

  • Mr. Anubrat Borah, Assam Agricultural University, Jorhat, Assam

    Project Associate, Directorate of Research(Agri), AAU, Jorhat

References

Ahmad KMS (2018) Genetic diversity of common bean (Phaseolus vulgaris L.) cultivars from different origins revealed by microsatellite markers. J. Adv. Biol.Biotechnol.17(4): 1-9.

Andrade RA, LM Lima and EV Rodrigues (2019) Genetic diversity and structure in common bean based on morphoagronomic traits. Genet. Resour. Crop Evol. 66(5): 1107-1121.

AOAC (2016) Official Methods of Analysis, 20th edn. Association of Official Analytical Chemists International, Gaithersburg, Maryland, USA.

Arriagada O, B Arévalo, RA Cabeza, B Carrasco and AR Schwember (2023) Meta-QTL analysis for yield components in common bean (Phaseolus vulgaris L.). Plants 12(1): 117.

Banoo A, N Ahmed, SH Khan and SM Zargar (2020) Genetic diversity assessment in common bean (Phaseolus vulgaris L.) germplasm using agro-morphological traits. Legume Res. 43(5): 674-680.

Beebe S, AV González and J Rengifo (2010) Research on trace minerals in the common bean. Food Nutr Bull 31(1 Suppl 1): 67-70.

Blair MW, LF González, PM Kimani and L Butare (2010) Genetic diversity, inter-gene pool introgression and nutritional quality of common bean (Phaseolus vulgaris L.) from Central Africa. Theor. Appl. Genet. 121(2): 237-248.

Broughton WJ, G Hernández, M Blair, S Beebe, P Gepts and J Vanderleyden (2003) Beans (Phaseolus spp.)-model food legumes. Plant Soil252(1): 55-128.

Celmeli T, H Sari, H Canci, D Sari, A Adak, T Eker and C Toker (2018) Nutritional content of common bean (Phaseolus vulgaris L.) landraces in comparison to modern varieties. Agronomy 8(9): 166.

Chahota RK, TR Sharma and SK Sharma (2020) Genetic variability and trait association studies in rajmash (Phaseolus vulgaris L.) under mid-hill conditions of Himachal Pradesh. Legume Res.43(6): 773-778.

Cichy KA, JA Wiesinger, M Berry, S Nchimbi-Msolla, D Fourie, TG Porch, D Ambechew and PN Miklas (2019) Role of genotype and environment in determining cooking time of dry beans (Phaseolus vulgaris L.). Legume Sci.1: e13.

De Paula E, RN Almeida, TO Santos, JDN Souza Neto, EMS RivaSouza, SCP Posse, MN Souza, AFF Madella de Oliveira, AC Santos Júnior and JO Santos (2024) Genetic diversity of common bean (Phaseolus vulgaris L.) landraces based on morphological traits and molecular markers. Plants 13(18): 2584.

Dutta M, A Saikia, N Gogoi and JD Nath (2015) Genetic variability studies in pole-type rajmah (Phaseolus vulgaris L.) genotypes of north-eastern hill region of India. Legume Res. 38(5): 631-635.

Fisher RA (1935) The Design of Experiments, 1st edn. Oliver and Boyd, Edinburgh, Scotland, 252 p.

Friendly M (2002) Corrgrams; exploratory displays for correlation matrices. The American Statistician56: 316-324.

Gelaw YM (2017) Genetic diversity in speckled bean (Phaseolus vulgaris L.) genotypes in Ethiopia. J. Plant Breed. Crop. Sci. 9(11): 200-207.

Girish M, G Muniswamy and S Ramesh (2021) Multivariate analysis for genetic divergence in rajmash (Phaseolus vulgaris L.) genotypes. J. Hortic. Sci.16(1): 47-54.

Gomez KA and AA Gomez (1984) Statistical Procedures for Agricultural Research, 2nd edn. John Wiley and Sons, New York, USA, 680 p.

Gu Z (2016) Complex heatmaps reveal patterns and correlations in multidimensional genomic data. Bioinformatics 32(18): 2847-2849.

Indian Farming (2017) Rajmah; an important pulse crop for hills. Indian Farming67(9): 17-19.

Jain A, P Sharma and V Kumar (2019) Morphological and biochemical characterization of common bean genotypes. Indian J. Genet. Plant Breed. 79(4): 737-744.

Jolliffe IT (2002) Principal Component Analysis, 2nd edn. Springer Verlag, New York, USA, 487 p.

Khan S, A Singh and M Sharma (2021) Nutritional and biochemical evaluation of common bean genotypes for protein and micronutrient content. Legume Res. 44(6): 673- 679.

Kwak M and P Gepts (2009) Structure of genetic diversity in the two major gene pools of common bean (Phaseolus vulgaris L.). Theor. Appl. Genet. 118: 979-992.

Long J, J Zhang, X Zhang, J Wu, H Chen, P Wang, Q Wang and C Du (2020) Genetic diversity of common bean germplasm resources based on morphological traits. Front. Genet. 11: 697.

Lowry OH, NJ Rosebrough, AL Farr and RJ Randall (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193(1): 265-275.

Mwale VM, JM Bokosi and MB Kwapata (2020) Morphological diversity of common bean landraces from Malawi. Int. J. Plant Breed. Genet. 14(1): 1-9.

Oliveira JS, MF Pereira and PA de Carvalho (2019) Genetic diversity of common bean based on morphological and biochemical traits. PesqAgropec Bras54: e00013.

R Core Team (2024) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna.

Rai N, M Rai and R Singh (2020) Characterization of common bean germplasm under North-Eastern hill conditions. Indian J. Agric. Sci.90(2): 378-383.

Raturi A, R Sharma and A Sharma (2017) Variability and correlation studies in common bean (Phaseolus vulgaris L.) genotypes. Legume Res.40(3): 464-469.

Shah AH, I Khaliq, G Bashir and S Jannat (2020) Genetic diversity in common bean germplasm of Jammu & Kashmir. Indian J. Agric. Res.54(5): 629-634.

Singh S, M Sood and A Sharma (2014) Genetic divergence and variability studies in rajmash (Phaseolus vulgaris L.). Legume Res.37(4): 439-444.

Singh Y, SK Yadav and S Sharma (2021) Multivariate genetic analysis of common bean genotypes. Plant Genet. Resour. 19(5): 432-440.

Sneath PHA and RR Sokal (1973) Numerical Taxonomy: The Principles and Practice of Numerical Classification. W.H. Freeman and Company, San Francisco, USA, 573 p.

Wickham H (2016) ggplot2: Elegant Graphics for Data Analysis, 2nd edn. Springer International Publishing, Cham, Switzerland, 260 p.

Downloads

Submitted

2026-01-02

Published

2026-08-11

How to Cite

Saikia, B., Borgohain, R., Goswami, G., Das, J., & Borah, A. (2026). Morpho-biochemical Characterization and Multivariate Assessment of Genetic Diversity in Rajmah (Phaseolus vulgaris L.) Genotypes from Northeast India: Genetic Diversity in Rajmah Genotypes. Indian Journal of Plant Genetic Resources, 39(2). https://doi.org/10.56093/ijpgr.v39i2.174765