Identification of Novel Compounds from Cissus quadrangularis L. by Gas Chromatography-Mass Spectrometry Analysis
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Authors
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Sidharth Tiwari
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Rajesh Saini
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Richa Upadhyay
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Ram Kant Ojha
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Saumya Mishra
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Dharm Raj Mishra
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Kavindra Nath Tiwari
Keywords:
Bone disorders, C. quadrangularis, GC-MS, Hadjod, Wound healingAbstract
Cissus quadrangularis(L.) commonly known as Asthisamharaka, Hadjod or Bonesetter, is a medicinal plant belonging to the family Vitaceae. The roots and stems of this plant have traditionally been used for the management of bone fractures and various musculoskeletal disorders, including osteoarthritis, rheumatoid arthritis and osteoporosis. In the present study, an ethanolic extract of C. quadrangularis stem was prepared and subjected to gas chromatography-mass spectrometry (GC-MS) analysis for the characterization of its phytoconstituents. The analysis resulted in the tentative identification of seventeen compounds. Based on an extensive literature survey and comparison with previously reported GC-MS profiles of C. quadrangularis, these compounds were not found to have been previously reported from the stem extract of this species. The physicochemical, drug-likeness and pharmacokinetic properties of the tentatively identified compounds were further evaluated using in silico ADMET analysis. Among the identified constituents, four compounds namely (5α)-Pregnane-3,20α-diol,14α,18α-[4-methyl-3-oxo-(1-oxa-4-azabutane-1,4-diyl)], [1,2,4] Triazolo[4,3-a] quinoxalin-4-amine, N-(2,3-dihydro-1,4-benzodioxin-6-yl),4H-Pyrido[1,2-a] pyrimidin-4-one, 6-methyl-3-phenyl-2,3-dihydroxypropyl(E)-Docos-13-enoate diacetate, and 2-Ethoxy-6,6-dimethyl-9-thioxo-6,9-dihydro-7,8-dithia-5a-azabenzothiazole-6-sulfonic acid, (4-methoxyphenyl) showed comparatively favourable predicted physicochemical and pharmacological characteristics, including parameters related to drug-likeness, absorption, distribution, solubility and toxicity. These compounds may therefore represent potential candidates for further pharmacological investigation. However, their
identities and biological activities require confirmation through isolation and structural characterization, followed by appropriate in vitro, in vivo and toxicological studies. The findings provide preliminary information on the phytochemical composition of C. quadrangularis stem and identify candidate constituents that warrant further investigation for their potential therapeutic relevance.
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