Screening of rice breeding lines for brown planthopper resistance using phenotypic and molecular methods


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Authors

  • B Kusuma Kumari et al

Keywords:

Rice, brown planthopper, resistance, screening, SSR markers

Abstract

One hundred and nineteen F 6 breeding lines of paddy along with resistant and susceptible checks were evaluated under feld and greenhouse conditions for brown planthopper (BPH), Nilaparvata lugens (Stal.) resistance during
Kharif, 2016 at APRRI & RARS, Maruteru. Out of which, 32 breeding lines in standard seed box screening and 69 in feld screening have exhibited resistant reaction to BPH. Twenty-fve breeding lines which exhibited resistance
reaction in both feld and seed box screenings along with resistant and susceptible checks were used for honeydew test and molecular screening. Among 25 breeding lines, MTU 2399-24-1-1-1 (13.67 mm2) and MTU 2465-11-3-1-1 (93.33 mm2) showed lower and higher honeydew excretion area, respectively. The molecular screening was done by using 33 SSR markers, which are linked to known BPH resistant loci. Out of 33 markers used, 20 markers
exhibited polymorphism and they classified the entries into II and III clusters using NTSYS and Darwin statistical packages, respectively.

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Submitted

2020-09-21

Published

2018-03-31

Issue

Vol. 46 No. 1 (2018)

Section

Articles

License

Manuscript will be accepted on the understanding that their content is original and that permission has been received in writing wherever necessary to produce previously published material (including quotations, data and illustrations) and that the manuscript has not been submitted/ accepted for publication elsewhere. Copyright resides with the Plant Protection Association of India.

How to Cite

Kumari et al, B. K. (2018). Screening of rice breeding lines for brown planthopper resistance using phenotypic and molecular methods. Indian Journal of Plant Protection, 46(1). https://epubs.icar.org.in/index.php/IJPP/article/view/104975