Association of recombinant cotton leaf curl begomovirus with higher Cotton leaf curl disease incidence in Delhi

Authors

  • V K Khare

Keywords:

Cotton, Cotton leaf curl-begomovirus, CLCuD incidence, phylogeny, recombination

Abstract

A recent survey of cotton leaf curl disease (CLCuD) in Indian Agricultural Research Institute (IARI), New Delhi cotton felds revealed higher CLCuD incidence of 60.0-70.2% in the year 2015 and 26.9-97.1% in the year 2016. Seven CLCuD-begomovirus isolates collected from infected felds were characterized based on sequence analysis of coat protein (CP) gene. The present isolates showed 94-100% nucleotide identity among them. The phylogenetic analysis determined that fve isolates are members of Cotton leaf curl Multan virus (CLCuMuV)-Rajasthan and two are of Cotton leaf curl Kokran virus (CLCuKoV)-Burewala. Recombination analysis among CP gene of overall 18 CLCuD begomovirus isolates of IARI cotton felds including the seven present and 11 previously reported isolates was conducted. Of them, 14 CLCuMuV-Rajasthan isolates, both the present and previously reported, were detected as recombinants showing break point at 192-584nt (P= 1.197 x 10-3-7.241 x 10-4) of the CP gene. Of the CLCuKoV-Burewala isolates, the present two isolates showed recombination at 629-740nt (P = 1.197 × 10-3) but the previously reported two isolates were non-recombinants. This is the frst report of appearance of recombinant CLCuKoV-Burewala isolate in Delhi. Although most of the CLCuD-begomoviruses in Delhi are recombinant, but the occurrence of higher CLCuD incidence at present condition might be due to appearance of recombinant CLCuKoV isolates.

Downloads

Download data is not yet available.

Downloads

Published

2018-03-31

How to Cite

Khare, V. K. (2018). Association of recombinant cotton leaf curl begomovirus with higher Cotton leaf curl disease incidence in Delhi. Indian Journal of Plant Protection, 46(1). Retrieved from https://epubs.icar.org.in/index.php/IJPP/article/view/104983

Issue

Section

Articles