Identification and characterization of Papaya ring spot virus - W isolate on bottlegourd in Telangana state of India


Abstract views: 41 / PDF downloads: 0

Authors

  • K Anitha
  • Y Prasanthi
  • Hameedunnisa Begum
  • S K Chakrabarty
  • P Sairam Reddy
  • B Sarath Babu

Keywords:

Bottlegourd, Papaya ringspot virus W, host range, characterization

Abstract

Incidence of Papaya ringspot virus (PRSV) was noticed in the F2 population of bottlegourd hybrid, “MGH 4-Waradâ€, grown in the experimental farm of Vegetable Research Station, Sri Konda Laxman Horticultural University, Rajendranagar, Hyderabad during 2013-2014. The disease was diagnosed and confi rmed by Direct antigen coated Enzyme linked immunosorbent assay (DAC-ELISA). Pathogenicity test resulted in symptom expression in watermelon and bottlegourd seedlings, but not in papaya indicating that the virus isolate under study is a cucurbit isolate (W) and not a papaya isolate (P) as it failed to infect papaya. Molecular characterization of the virus isolate revealed the presence of amplicon of ~770 bp in the infected samples, while none in healthy sample. The test isolate was identified as a potyvirus, namely PRSV-Watermelon strain and is closely related to PRSV-W isolates of watermelon and pumpkin from Karnataka with 98.7 and 96.6 per cent identity, respectively and cucumber isolate from Tamilnadu with 96.3 per cent identity.

Downloads

Submitted

2020-09-21

Published

2017-12-31

Issue

Section

Articles

How to Cite

Identification and characterization of Papaya ring spot virus - W isolate on bottlegourd in Telangana state of India. (2017). Indian Journal of Plant Protection, 45(4). https://epubs.icar.org.in/index.php/IJPP/article/view/105018