Cloning and expression of the vegetative insecticidal protein gene of Bacillus thuringiensis in Escherichia coli and its use in bioassay


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Authors

  • Gunjan Bharti
  • P U Krishnaraj

Keywords:

vip3A, Bacillus thuringiensis, Escherichia coli, Plutella xylostella

Abstract

Amplicon restriction fragment length polymorphism (ARFLP) method was used for the identification of a variant of vip3 gene. The variant vip3 related gene was identified in Bacillus thuringiensis. This variant gene is 2370 bp long and codes for a 91-kDa protein (801 amino acids). The amino acid sequence exhibited 99 per cent sequence similarity with vip3Aa. The vip3Aa protein was produced in Escherichia coli and tested against the third instar larvae of Plutella xylostella, where it caused 96.67 per cent mortality.

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Submitted

2020-09-26

Published

2020-09-30

How to Cite

Bharti, G., & Krishnaraj, P. U. (2020). Cloning and expression of the vegetative insecticidal protein gene of Bacillus thuringiensis in Escherichia coli and its use in bioassay. Indian Journal of Plant Protection, 45(2). Retrieved from https://epubs.icar.org.in/index.php/IJPP/article/view/105236

Issue

Vol. 45 No. 2 (2017)

Section

Articles

License

Manuscript will be accepted on the understanding that their content is original and that permission has been received in writing wherever necessary to produce previously published material (including quotations, data and illustrations) and that the manuscript has not been submitted/ accepted for publication elsewhere. Copyright resides with the Plant Protection Association of India.