Genetic diversity and structure of pearl millet infecting populations of Magnaporthe grisea
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Keywords:Blast, Magnaporthe grisea, Pennisetum glaucum, Universal Rice Primers (URPs)
AbstractGenetic diversity and structure of pearl millet infecting population of Magnaporthe grisea was studied usingÂ Universal Rice Primers (URPs). Sixty fve isolates of M. grisea from pearl millet and fve isolates from other hostsÂ were used in this study. Nine URP primers (URP-1F, 2F, 4R, 6R, 9F, 17R, 25F, 30F and 38F) produced 105 easilyÂ scorable amplifed fragments. The polymorphic information content for URPs varied from 0.32 to 0.50 with anÂ average of 0.43. Cluster analysis separated the isolates into fve main clusters. Analysis of molecular varianceÂ (AMOVA) revealed that 16% of the genetic variation was due to difference between populations of M. griseaÂ adapted to pearl millet and non-pearl millet hosts, and 84% variation was due to difference in the isolates. TheÂ results of population structure analysis revealed that isolates were grouped in different clusters according to theirÂ genetic relatedness irrespective of their host origin as well as geographical distribution.
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