Lethal and sub lethal effects of Metarhizium anisopliae against Spodoptera litura
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Keywords:Metarhizium anisopliae, entomopathogenic fungi, Spodoptera litura, local isolates.
AbstractThe effect of Metarhizium anisopliae was determined against Spodoptera litura by leaf dip method. A totalÂ of three isolates (SSB, SBvB and SBvD) of M. anisopliae from 60 samples soil and insect samples wereÂ obtained based on their pathogenicity test against S. litura. Among the three isolates, SSB (76.67 %) andÂ SBvB (63.33 %) showed highest mortality whereas the least mortality was found in the isolate SBvD (50.00Â %) at six days after treatment at the concentration of 3x 108 cfu/1000 ml. Further, sub lethal effect wereÂ also studied and showed that, the per cent pupation, adult emergence and fecundity (23.33, 6.67, 0.00, respectively) were very poor in the SSB isolate depicting the high virulence whereas SBvB isolate recordedÂ 50.00, 23.33, 23.00 per cent, respectively indicating poor virulence. The order of mortality and sub lethalÂ effect of these isolates were SSB>SBvB>SBvD. These isolates performed better under laboratory conditionsÂ and need to be evaluated under field conditions for their promotion against soil dwelling and defoliatingÂ insect pests of this geographical area
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