Marker assisted introgression of broad spectrum blast resistance gene Pi-2 into an elite rice cultivar, Samba Mahsuri
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Keywords:Pyricularia oryzae, Samba Mahsuri, C101A51, MAI, blast resistant gene
AbstractRice blast disease is caused by fungal pathogen Pyricularia oryzae is one of the most devastating cropÂ disease worldwide and its effects on rice production up to 70-80% in favourable conditions. In recentÂ years, the rice scientists mainly concentrated on to develop new varieties for increasing production andÂ productivity of rice with durable resistance to abiotic and biotic stress. In present study, broad spectrumÂ rice blast resistant gene Pi-2 originating in the isogenic line C101A51 was incorporated into an eliteÂ cultivar â€˜Samba Mahsuri (BPT 5204)â€™, which is highly susceptible to blast disease through one of the trendyÂ breeding programme i.e., Marker Assisted Introgression (MAI). It involved backcross and selfing, at eachÂ backcross foreground selection was carried out by using gene specific linked marker MSM1. As a result,Â background selection was done by using a set of 61 parental polymorphic SSR markers spread across theÂ rice genome. At BC1F1 a single plant possessing targeted gene Pi-2 along with maximum recovery ofÂ recurrent parent genome (~ 75.40%; plant # BA-23-11) was selected and advanced through selfing andÂ ancestry-based selection for agro-morphological traits. Results confirmed that, resistance gene Pi-2 wasÂ successfully incorporated into recurrent parent Samba Mahsuri and three lines, viz., BA-23-11-89-12-56,Â BA-23-11-89-12-96 and BA-23-11-89-12-168 were identified at BC2F4 which are possessing high level ofÂ resistance against blast disease with high virulent of P. oryzae isolate (IIRR MSP-28). The agronomicalÂ traits of â€˜ABLâ€™sâ€™ were very similar to those of the recurrent parent Samba Mahsuri. One ABL (BA-23-11-Â 89-12-96; ~ 93.40%) was found to be better than recurrent parent Samba Mahsuri (BPT 5204) regardingÂ resistance and yield. The application of MAI is very useful to develop blast resistant variety with highestÂ recovery of recurrent parent genome and also to reduce number of generations and time.
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