Serological assay of Tobacco streak virus (TSV) in the different plant parts of germplasm and advance generations of Gossypium barbadense


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Authors

  • P Valarmathi Valarmathi
  • K P M Dhamayanthi

Keywords:

Tobacco Streak Virus (TSV), Gossypium barbadense, DAS-ELISA.

Abstract

Tobacco streak virus (TSV), the causal agent of cotton necrosis, is an emerging disease of national significance in India. Tobacco streak virus (TSV), inciting cotton necrosis, exhibits multifarious symptoms which includes purplish brown, necrotic lesions in the leaves, squares, and petioles. Development of diagnostic tools with rapidity will have immense role to play in detection and management of the emerging virus. A simple and rapid procedure of enzyme immunoassay (ELISA) was used to detect and identify viruses in individual plants. Serological assay by double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA) revealed the presence of TSV in leaf, square, stem, root, petiole and pollen grains of germplasm and advance generations of Gossypium barbadense.

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Submitted

2021-04-06

Published

2021-04-06

Issue

Vol. 48 No. 3 (2020)

Section

Articles

License

Manuscript will be accepted on the understanding that their content is original and that permission has been received in writing wherever necessary to produce previously published material (including quotations, data and illustrations) and that the manuscript has not been submitted/ accepted for publication elsewhere. Copyright resides with the Plant Protection Association of India.

How to Cite

Valarmathi, P. V., & Dhamayanthi, K. P. M. (2021). Serological assay of Tobacco streak virus (TSV) in the different plant parts of germplasm and advance generations of Gossypium barbadense. Indian Journal of Plant Protection, 48(3). https://epubs.icar.org.in/index.php/IJPP/article/view/111530