Pre and post evaluation of direct antigen coating - enzyme-linked immuno sorbent assay (DAC-ELISA) for the detection of BBrMV and CMV in micropropagation of banana

Abstract

Plantains (Musa spp.) cv. Kovvur Bontha are one of the largest fruit crops produced mostly by the developing countries in the tropical and subtropical regions. Major propagule in banana cultivation is its vegetative suckers and the availability of uniform and disease-free suckers is the main problem faced by the farmers. The use of tissue culture planting material solved this problem to a greater extent by giving uniform growth and production. But there is no assurance for disease free material. Direct Antigen Coating Enzyme Linked
Immuno Sorbent Assay (DAC-ELISA), a serological method was ussed to detect the presence of viral proteins. The main principle of DAC-ELISA is the detection of presence of viral protein through interaction between antigen from the coat protein with the rabbit anti-bodies. The outcome of the experiment shows
that after eradication of the viral infected material at early stage will help in the production of viral free planting material. DAC-ELISA was the cheapest and easiest method to detect the virus so that we can get the good and disease-free planting material.