Detection, identification and molecular characterization of phytoplasma associated with sesame phyllody and leafhopper in Delhi, India

Authors

  • Pardeep Kumar Division of Plant Quarantine, ICAR-National Bureau of Plant Genetic Resources, New Delhi - 110 012, India.
  • V Celia Chalam Division of Plant Quarantine, ICAR-National Bureau of Plant Genetic Resources, New Delhi - 110 012, India.
  • T Boopathi Division of Crop Protection, ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad - 500 030, India.
  • B Parameswari ICAR-National Bureau of Plant Genetic Resources, Regional Station, Hyderabad - 500 030, India.
  • Hemavati Ranebennur Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi - 110 012, India.
  • S C Dubey Assistant Director General (PP&B), Indian Council of Agricultural Research, Krishi Bhavan, New Delhi - 110 001, India.

Keywords:

Sesame, phyllody, nested PCR, phytoplasma, leafhopper.

Abstract

The phyllody symptomatic plants were found in sesame fields in Delhi (India) during Kharif 2018. A nested polymerase chain reaction using phytoplasma universal primers P1/P7 followed by R16F2n/R16R2 was used to detect phytoplasmas in symptomatic plants and leafhopper (Orosius albicinctus). The amplified 16S rDNA fragment was sequenced and BLAST analysis of 1.2-kb fragment confirmed the presence of
phytoplasma in infected plants and leafhoppers. The virtual RFLP analysis of the amplified DNA fragments using 17 restriction enzymes showed that the detected phytoplasma belonged to the 16SrI-B group (Candidatus Phytoplasma asteris). The phylogenetic analysis showed that newly characterized isolate NBPGR-1 clustered with previously identified 16Sr I-B of sesame phyllody along with other 16Sr I-B group
phytoplasma. 

Additional Files

Published

2024-07-01

Issue

Vol. 51 No. 1,2&3 (2023): Indian Journal of Plant Protection

Section

Articles

License

Manuscript will be accepted on the understanding that their content is original and that permission has been received in writing wherever necessary to produce previously published material (including quotations, data and illustrations) and that the manuscript has not been submitted/ accepted for publication elsewhere. Copyright resides with the Plant Protection Association of India.

How to Cite

Kumar, P., Celia Chalam, V., Boopathi, T., Parameswari, B., Ranebennur, H., & Dubey, S. C. (2024). Detection, identification and molecular characterization of phytoplasma associated with sesame phyllody and leafhopper in Delhi, India. Indian Journal of Plant Protection, 51(1,2&3), 44-49. https://epubs.icar.org.in/index.php/IJPP/article/view/153298