Screening of blackgram genotypes for infective analysis of MYMIV and MYMV associated with three blackgram isolates using agroinoculation technique
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Authors
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D Vijay Kumar Naik
Regional Agricultural Research Station, Acharya N. G. Ranga Agricultural University, Tirupati - 517 502, India.
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B V Bhaskara Reddy
Regional Agricultural Research Station, Acharya N. G. Ranga Agricultural University, Tirupati - 517 502, India.
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R Sarada Jayalakshmi Devi
Sri Venkateswara. Agricultural College, Acharya N. G. Ranga Agricultural University, Tirupati - 517 502, India.
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L Prasanthi
Regional Agricultural Research Station, Acharya N. G. Ranga Agricultural University, Tirupati - 517 502, India.
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Lakshminarayana R Vemireddy
Sri Venkateswara. Agricultural College, Acharya N. G. Ranga Agricultural University, Tirupati - 517 502, India.
Keywords:
Agrobacterium transformation, infectious clones, MYMV, MYMIV, blackgram and agroinoculation.Abstract
The infectious dimeric clones of two species of begomoviruses, mungbean yellow mosaic India virus (MYMIV) and mungbean yellow mosaic virus (MYMV) of three isolates (East Godavari, Kurnool and Prakasam) were constructed by ligating partial digested product of RCA at BamHI site of pCAMBIA 2301vector. Ligated product was transformed into E. coli cells. The infectivity analysis of constructed dimeric clones of MYMIV
and MYMIV of three isolates were done by sprouted seed method of agroinoculation using susceptible (LBG645 and PBG32) and resistant (PU31 and TBG104) blackgram genotypes. Among the three isolates tested for both the species of begomoviruses, maximum PDI was observed with East Godavari isolate as compared to Kurnool and Prakasam isolates. Screening of different blackgram genotypes screened for resistance to MYMIV and MYMV using agroinoculation technique revealed that YMV isolate collected from East Godavari district was more virulent than YMV isolates collected from Kurnool and Prakasam districts of Andhra Pradesh.
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