Molecular profiling of native Trichoderma asperellum and Pseudomonas fluorescens from tomato rhizosphere in Arunachal Pradesh
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Authors
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Raghuveer Singh
ICAR Research Complex for NEH Region, Arunachal Pradesh Centre, Basar-791101, Arunachal Pradesh, India.
Keywords:
Tomato, Trichoderma, Pseudomonas, ITS region, phylogenetic analysisAbstract
The present study aimed to identify and characterize potent native bioagents from the tomato rhizosphere with strong antagonistic potential against Phytophthora infestans, the causal agent of late blight in tomato. In vitro screening revealed the highest growth inhibition by Pseudomonas fluorescens isolates Pf-2 (81.33%) and Pf-3 (73.33%), followed by Trichoderma asperellum isolates T-11 (73.73%) and T-14
(66.67%). Molecular characterization of fungal isolates (T-11 and T-14) using the ITS region of the 18S rRNA confirmed 100% similarity with T. asperellum (GenBank: MK928414, MK928417), clustering them phylogenetically with T. asperellum. Similarly, rhizospheric bacterial isolates (Pf-2 and Pf-3) were characterized using 16S-23S rRNA ITS1 and ITS2 primers, amplifying a 560 bp fragment. Phenotypic characterization further identified them as P. fluorescens. These findings emphasize the importance of precise taxonomic identification to ensure microbial safety in the rhizosphere. The identified T. asperellum and P. fluorescens isolates exhibit promising biocontrol potential and warrant further exploration for
sustainable tomato disease management.
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Manuscript will be accepted on the understanding that their content is original and that permission has been received in writing wherever necessary to produce previously published material (including quotations, data and illustrations) and that the manuscript has not been submitted/ accepted for publication elsewhere. Copyright resides with the Plant Protection Association of India.