Concurrent infection of Pneumomycosis, verminous enteritis and Staphylococcal blepharitis in native chicken farm

Abstract

Two 8-week old Aseel chicks were presented for necropsy from a native chicken farm with the history of swollen left eyelid, dyspnoea, off feed, dullness and sudden death. Gross examination of the carcasses revealed swollen left eye with cheesy exudate on incision. Lungs revealed severe congestion, with multifocal grey consolidated areas (1-2 mm diameter) while, the intestinal lumen was clogged by numerous round worms. Histopathological examination of lungs revealed multifocal granulomatous area with central necrosis, presence of macrophages, lymphocytes and multinucleated giant cells. Special staining with Periodic acid Schiff and Grocott-Gomori’s methenamine silver stain showed the presence of fungal hyphae and spores in the lung lesions. The fungus was identified as Aspergillus sp. by cultural examination. Intestines revealed numerous cut sections of worms with eosinophilic and mononuclear cell infiltration and were identified as Ascaridia galli. Eye lesion had multifocal mild to moderate mononuclear cell infiltration with osseous metaplasia of scleral cartilage in eyeball. Staphylococcus hyicus was isolated from the eye exudate by cultural examination. Etiological diagnosis revealed an outbreak of Aspergillosis, Ascariasis and Staphylococcal blepharitis in the farm.