Immunochemical localization of cross-reactive proteins of bladderworms with special reference to detection of specific antigen in cystic echinococcosis of sheep
Immunochemical localization of cross-reactive proteins of ----cystic echinococcosis of sheep
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Authors
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Jeyathilakan, N
TANUVAS
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Abdul Basith, S
TANUVAS
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Lalitha John
TANUVAS
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Daniel Joy Chandran, N
TANUVAS
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Dhinakarraj, G
TANUVAS
Keywords:
Antigens, Coenurus cerebralis, Cysticercus tenuicollis, Hydatid cysts, Hydatid cysts, SDS PAGE, Sheep, Western blotAbstract
Immunochemical localization of antigenic fractions of ovine bladder worms viz., hydatid cyst, Coenurus cerebralis and Cysticercus tenuicollis is important to identify cross reactive as well as specific proteins. SDS PAGE and western blot technique were carried out to analyze various portions of three ovine bladderworms and to identify the specific protein of cystic echinococcosis. SDS PAGE analysis of fluid antigens revealed 9,6 and 9 bands for hydatid cyst(HCFA),Coenurus cerebralis(CCFA)and Cysticercus tenuicollis(CTFA) respectively and 24, 38 and 68 kDa protein bands were common in HCFA and CCFA. The common protein band between HCFA and CTFA was identified as 28kDa.24 kDa protein which was common between CCFA and CTFA. Scolex antigen revealed 3,6 and 9 bands respectively for HPSA, CCSA and CTSA. The common protein bands between HPSA and CTSA were 29, 72 and 98 kDa. Protein bands 12, 42,98 and 112 kDa were common between CCSA and CTSA. Membrane antigen revealed 4, 4 and 6 bands respectively for HGMA, CCMA and CTMA. The common protein band between CCMA and CTMA was 16kDa only. Western blot analysis revealed that the low molecular weight protein 8 kDa from HCFA was specific for cystic echinococcosis. Cross reaction was noticed between HPSA and CTMA as well as between HGMA and CTMA..
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