Superovulation and Embryo Yield after GNRH Pretreatment in Crossbred Cows
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Authors
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S. Satheshkumar
Department of Animal Biotechnology, Madras Veterinary College Tamilnadu Veterinary and Animal Sciences University (TANUVAS), Chennai — 600 007
Keywords:
GnRH pretreatment, superovulatory response, embryo yield, crossbred COWSAbstract
The effect of GnRH pretreatment on superovulatory response (SOR) and embryo yield in crossbred cattle was studied. Six crossbred cows were subjected to three superovulatory treatments: i. Control: Four day FSH schedule was initiated on Day 10 of the cycle ii. Gn-D8: GnRH was administered on Day 6 and FSH schedule was initiated on Day 8 and iii. GnD10: GnRH was administered on Day 6 and FSH schedule was initiated on Day 10. In control group, the mean SOR was 13.7 + 5.8 CL. In Gn-D8 group, the SOR is inconsistent (5.0 ± 1.7) with recovery of poor quality embryos, which was attributed to significantly higher progesterone concentration on the day of superovulatory oestrus. In Gn-D10 group, SOR (11.0 ± 1.6) and embryo yield were comparable to the control group. Transferable quality embryos were signcantly higher in Gn-D10 group which could be attributed to the follicular maturation under favourable endocrine environment. Thus, GnRH pretreatment in superovulation protocol ensured consistent SOR and increased yield of transferable quality embryos in crossbred cattle.
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Abdelatty, A.M., Iwaniuk, M.E., Potts, S.B and Gad, A. (2018) Influence of maternal nutrition and heat stress on bovine oocyte and embryo development. International Journal of Veterinary Science and Medicine, 6:S1-S5
Adams, G.P., Nasser, L.F., Bo, G.A., Garcia, A and Del Campo, M.R. (1994). Superovulatory response of ovarian follicles of Wave 1 versus Wave 2 in heifers. Theriogenology, 42:1103-13.
Deyo, C.D., Colazo, M.G., Martinez, M.F and Mapletoft, R.J. (2001). The use of GnRH or LH to synchronize follicular wave emergence for superstimulation in cattle. Theriogenology, 55:513.
D'Occhio, M.J., Sudha, G., Jillella, D., Whyte, T and Maclellan, L.J. (1997). Use of a GnRH agonist to prevent the endogenous LH surge and injection of exogenous LH to induce ovulation in heifers superstimulated with FSH: A new model for superovulation. Theriogenology, 47:601-613.
Driancourt, M. A. (2001). Regulation of ovarian follicular dynamics in farm animals. Implication for manipulation of reproduction. Theriogenology, 55:1211-39.
Duchens, M., Forsberg, M., Edqvist, L.E., Gustafsson, H and Martinez, H.R. (1994). Effects of induced suprabasal progesterone levels around oestrus on plasma concentrations of progesterone, estradio1-17f3 and LH in heifers. Theriogenology, 42:1159-69.
Farin, P.W., Dowdall, K.M., Hicks, J.E., Farin, C.E and Whishant, C.S. (2008). Subcutaneous administration of follicle stimulating hormone for superovulation of Holstein cows. Reproduction Fertility and Development, 21:243-244.
Fortune, J.E., Rivera, G.M., Evans, A.C.O and Turzillo, A.M. (2001). Differentiation of dominant versus subordinate follicles in cattle. Biology of Reproduction, 65:648-654.
Ginther, 0.J., Beg, M.A., Donadeu, F.X and Bergfelt, D.R. (2003). Mechanism of follicle deviation in monovular farm species. Animal Reproduction Science, 78:239-257.
Kathiresan, D., Palanisamy, A., Selvaraju, M and Nachimuthu, K. (1997). Influence on time of onset of estrus on embryo recovery in superovulated crossbred cows. Indian Journal of Animal Reproduction, 18:6-7.
Kohram, H., Twagiramungu, H., Bousquet, D., Durocher, J and Guilbault, L.A. (1998). Ovarian superstimulation after follicular wave synchronization with GnRH at two different stages of the oestrous cycle in cattle. Theriogenology, 49:1175-86.
Lee, W.Y., Song, K.Y., Lim, K.T., Lee, S.J and Lee, B.C. (2012). Influence of factors during superovulation on embryo production in Korean Holstein cattle. Journal of Veterinary Medicine Science, 74:167-174.
Lindner, G.M and Wright, R.W. (1983). Bovine embryo morphology and evaluation. Theriogenology, 20:407¬416.
Mapletoft, R.J., Bo, G.A and Baruselli, P.S. (2009). Control of ovarian function for assisted reproductive technologies in cattle. Animal Reproduction, 6:114¬124.
Nasser, L.F., Adams, G.P., Bo, G.A and Mapletoft, R.J. (1993). Ovarian superstimulatory response relative to follicular wave emergence in heifers. Theriogenology, 40:713-724.
Rajamahendran, R and Calder, M.D. (1993). Superovulatory responses in dairy cows following ovulation of the dominant follicles of the first wave. Theriogenology, 40:99-109.
Satheshkumar, S., Palanisamy, A.,Ramadass, P., Subramanian, A and Kathiresan, D. (2008). Follicular wave synchronization using GnRH agonist in Jersey crossbred cows. Indian Journal of Animal Reproduction, 8:154-158.
Satheshkumar, S., Subramanian, A., Devanathan, T.G., Kathiresan, D., Veerapandian, C and Palanisamy, A. (2012). Follicular and endocrinological turnover associated with GnRH induced follicular wave synchronization in Indian crossbred cows. Theriogenology, 77:1144-50.
Sato, T., Nakada, K., Uchiyama, Y., Kimura, Y and Fujiwara, N. (2005). The effect of pretreatment with different doses of GnRH to synchronize follicular wave on superstimulation of follicular growth in dairy cattle. Journal of Reproduction and Development, 51:573-578.
Snedecor, G.W and W.G.Cochran, 1994. Statistical methods (8th ed). Iowa State University Press, USA.
Vieira, L.M., Rodrigues, C.A., Mendanha, M.F., Sa Filho, M.F and Sales, J.N.S. (2014). Donor category and seasonal climate associated with embryo production and survival in multiple ovulation and embryo transfer programs in Holstein cattle. Theriogenology, 82:204-212.
Yadav, M.C., Waltona, J.S and Leslie, K. (1986). Plasma concentrations of luteinizing hormone and progesterone during superovulation of dairy cows using follicle stimulating hormone or pregnant mare serum gonadotrophin. Theriogenology, 26:523-540.
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