PROTECTIVE EFFECTS OF RAW AND NANO FORMS OF RESVERATROL AND SILIBININ IN DIETHYL NITROSAMINE  INDUCED HEPATIC DAMAGE IN WISTAR RATS

J. Venkatesh Yadav; G. V. Sudhakar Rao; N. Pazhanivel; G. Sarathchandra; T. M.A Senthil Kumar

doi:10.56093/ijvasr.v52i1.137542

Authors

J. Venkatesh Yadav Ph.D. Scholar, Department of Veterinary Pathology, Madras Veterinary College, TANUVAS, Chennai- 600 007
G. V. Sudhakar Rao Professor and Head, Department of Veterinary Pathology, Madras Veterinary College, TANUVAS, Chennai- 600 007
N. Pazhanivel Professor, Department of Veterinary Pathology, Madras Veterinary College, TANUVAS, Chennai- 600 007
G. Sarathchandra Professor and Head, PLAFFS , TANUVAS, MMC, Chennai - 600 051
T. M.A Senthil Kumar Professor and Head, Zoonosis Research Laboratory, TANUVAS, Chennai - 600 051

https://doi.org/10.56093/ijvasr.v52i1.137542

Keywords:

Resveratrol, silibinin, diethyl nitrosamine, antioxidants, solid lipid nanoparticles

Abstract

Oxidative stress is a common mechanism contributing to initiation and progression of hepatic damage in a variety of liver disorders. Hence, there is a great demand for the development of agents with potent antioxidant effect. The aim of the present investigation is to evaluate the efficacy of raw and nanoforms of resveratrol and silibinin as a hepatoprotective and an antioxidant against diethylnitrosamine induced hepatocellular damage. 0.01% diethylnitrosamine administration to rats resulted in significantly elevated levels of lipid peroxidation (LPO) and decreased levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GSH) and total protein (TP) in the liver tissue. Post-treatment with the nano resveratrol (50 mg/kg), nano silibinin (50 mg/kg), raw resveratrol (50 mg/kg), raw silibinin (50 mg/kg) orally for 30 days significantly reversed the diethylnitrosamine induced alterations in the liver tissue and offered almost complete protection. The results from the present study indicate that both nano forms exhibit good hepatoprotective and antioxidant potential against diethylnitrosamine induced hepatocellular damage in rats as compared to raw forms.

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