FATTY ACID METHYL ESTER PROFILE OF THE LIPOPOLYSACCHARIDES FROM SELECTED SEROVARS OF  PATHOGENIC LEPTOSPIRA

Sowndarya Murali; K.G. Tirumurugaan; K. Senthil Kumar; V. Vinitha; S. Meignanalakshmi; Ghadevaru Sarathchandra; B. Samuel Masilamoni Ronald; R. P. Aravindh Babu

doi:10.56093/ijvasr.v52i1.137557

Authors

Sowndarya Murali Ph. D. Scholar, Department of Animal Biotechnology, Madras Veterinary College, TANUVAS, Chennai-7
K.G. Tirumurugaan Project Director i/c, Translational Research Platform for Veterinary Biologicals, Centre for Animal Health Studies, TANUVAS, Chennai- 600 051
K. Senthil Kumar Associate Professor, Department of Veterinary Microbiology, VCRI, Salem
V. Vinitha Ph. D. Scholar, Department of Animal Biotechnology, Madras Veterinary College, TANUVAS, Chennai-7
S. Meignanalakshmi Professor, Department of Animal Biotechnology, Madras Veterinary College, TANUVAS, Chennai-7
Ghadevaru Sarathchandra Professor and Head, Pharmacovigilance Laboratory For Animal Feed and Food Safety (PLAFFS), Centre for Animal Health Studies, TANUVAS, Chennai-51
B. Samuel Masilamoni Ronald Professor, Department of Veterinary Microbiology, Madras Veterinary College, TANUVAS, Chennai – 7
R. P. Aravindh Babu Assistant Professor and Scientist, Translational Research Platform for Veterinary Biologicals, Centre for Animal Health Studies, Tamil Nadu Veterinary and Animal Sciences University, Chennai- 600 051

https://doi.org/10.56093/ijvasr.v52i1.137557

Keywords:

MAT, sero-reactivity, cross-serovar reactivity, LPS

Abstract

Leptospirosis is one of the most important zoonotic disease caused by the pathogenic leptospires Leptospira interrogans. In this study, four pathogenic Leptospira serovars were selected based on the seroprevalence data, their LPS extracted, assessed for its purity and seroreactivity and processed for the determining the fatty acid profiles. The average concentration of the LPS extracted from the cultures across the serovars was 742.75+19.4 µg/ml with the polysaccharides contributing to 77.2 % of the LPS (573.6+18.01 µg/ml). The common fatty acids that were present in all the four serovars included butyric acid (C4:0), capric acid (C10:0), palmitoleic acid (C16:0), eicosapentaenoic acid (C20:5n3) and docosapentaenoic acid (C22:6n3). The heptadecanoic acid (C17:1) was present in serovars australis and autumnalis; pentadecanoic acid (C15:0) in canicola and hardjo serovars and caproic acid (C6:0) in the serovars autumnalis and canicola. The fatty acid eicosatrinoic acid (C20:3n3) was unique to the serovar autumnalis and the myristic (C13:0) and docosadienoic acid (C22:2) being unique to the serovar hardjo. Future studies to understand the fatty acid profile of the other common pathogenic circulating Leptospira LPS will add scientific evidence to the minor cross-reactivity observed across the serovars in MAT.

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