A NOVEL SUPEROVULATORY PROTOCOL fOR ENHANCEMENT Of BOVINE IN VIVO EMBRYO YIELD

D. Reena; D. Gopikrishnan; S. Rangasamy; S. Balasubramanian

doi:10.56093/ijvasr.v47i3.137895

Authors

D. Reena Assistant Professor, Department of Animal Biotechnology, Madras Veterinary College, Chennai –7
D. Gopikrishnan Assistant Professor, Resident Veterinary Services Section, Madras Veterinary College, Chennai –7
S. Rangasamy Assistant Professor, Department of Veterinary Gynaecology & Obstetrics, Madras Veterinary College, TANUVAS, Chennai - 600 007
S. Balasubramanian Director of Clinics i/c, Madras Veterinary College, TANUVAS, Chennai - 600 007

https://doi.org/10.56093/ijvasr.v47i3.137895

Keywords:

Superovulation, In vivo embryos, Synchronization and Embryo transfer

Abstract

The aim of this study was to evaluate two different superovulation protocols for quality embryo production. Donor animals were selected (Day 0) and a progesterone device was inserted. The superstimulatory treatment was initiated in day 7 (D7) with follicle stimulating hormone from porcine (FSHp) in eight decreasing doses each at 12 hours interval. Along with the fifth dose of FSHp, 500μg cloprostenol was administered. The progesterone device was removed 12h (Treatments P12) and 24 h after the first application of cloprostenol (Treatments P24). Twelve hours after the eighth dose of FSHp (D11) 25µg of Buserelin was administered and the inseminations were done after 12 and 24 hours. Embryos were collected in the forenoon of D18. P24 presented better results than the P12 treatment.

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References

Baruselli, P.S., Filho, M.F.S., Martins, C.M., Nasser, L.F., Noguiera, M.F.G., Barros, C.M. and Bo, G.A. (2006). Superovulation and embryo transfer in Bos indicus cattle. Thereogenology, 65: 77- 88.

Bo, G.A., Baruselli, P.S., Moreno, D., Cutaia, L., Caccia, M. and Trıbulo, R. (2002). The control of follicular wave development for self appointed embryo transfer programs in cattle. Theriogenology, 57: 53- 72.

Carvalho, J.B., Carvalho, N.A., Reis, E.L., Nichi, M., Souza, A.H. and Baruselli, P.S. (2008). Effect of early luteolysis in progesterone-based timed AI protocols in Bos indicus x Bos taurus heifers. Theriogenology, 69: 167-175.

Silva, C.J., Lopes da Costa, L. and Robalo Silva, J. (2002). Plasma progesterone profiles and factors affecting embryo fetal mortality following embryo transfer in dairy cattle. Theriogenology, 58: 51- 59.

Nasser, L.F., Reis, E.L., Oliveira, M.A., Bo, G.A. and Baruselli, P.S. (2004). Comparison of four synchronization protocols for fixed- time bovine embryo transfer in Bos indicus x Bos Taurus recipients. Theriogenology, 62: 1577-1584.