A pilot study on cryopreservation of canine platelets
51 / 0
Authors
-
M. Ranjith Kumar
M.V.Sc Student, Department of Veterinary Clinical Medicine, Madras Veterinary College, TANUVAS, Chennai - 7
-
P. Selvaraj
Department of Veterinary Clinical Medicine, Madras Veterinary College, TANUVAS, Chennai - 7
-
S. Prathaban
Professor and Head, Department of Veterinary Clinical Medicine, Madras Veterinary College, TANUVAS, Chennai - 7
-
G. Dhinakar Raj
Translational Research Platform for Veterinary Biologicals, TANUVAS, MMC, Chennai - 600 051
-
V. Maroudam
Referral Centre for Veterinary Transfusion Medicine, Veterinary University Peripheral Hospital, TANUVAS, MMC, Chennai - 51
Keywords:
Canine platelets, cryopreservation , platelet count and recoveryAbstract
Platelet transfusion is less commonly used in veterinary medicine, since the platelet products are not readily available. Cryopreservation of canine platelets may provide ready to use platelets. Towards this, a study was undertaken to assess cryopreservation of canine platelets for long term usage. Platelets were collected from healthy donors were then divided into three categories: fresh, cryopreserved in 6 percent Dimethyl Sulfoxide (DMSO) and cryopreserved in 6 percent Hydroxy Ethyl Starch (HES). After adding cryo-protectant to platelets, they were transferred to cryocyte containers and placed in a -80˚C freezer and stored. Assessment of the platelet count and recovery was carried out on 7 th and 14 th day of cryopreservation. Aerobic bacterial cultures from all tested platelet units yielded no growth. The use of DMSO as a cryo-protectant with a controlled cooling rate was found to be a simple, rapid and efficient method for canine platelet cryopreservation and provided a good recovery of platelets than the use of HES.
Downloads
References
Alving, B., T. Reid and Fratantoni, J.C. (1997). Frozen platelets and platelet substitutes in transfusion medicine. Transfusion, 37: 866-876.
Appleman, E.H., Sachais, B.S., Patel, R., K.J. Drobatz,Groman R.P., Kennedy D.R, O’Donnell, P.A. Bryan, C. and M.B. Callan (2009). Cryopreservation of Canine Platelets. J Vet Intern Med 2009, 23: 138-145.
Balint, B., Paunovic, D., Vucetic, D. (2006). Controlled-rate versus uncontrolled-rate freezing as predictors for platelet cryopreservation efficacy. Transfusion, 46: 230-235.
Brecher, M.E. ed. (2005). American Association of Blood Banks Technical Manual, 15th ed. Bethesda: AABB.
Callan, M.B., Appleman, E.H. and Shofer, F.S. (2008). Clinical and Clinicopathologic effects of plateletpheresis on healthy donor dogs. Transfusion, 48: 2214-2221.
Dijkstra-Tiekstra, M.J., Kuipers, W., Setroikromo, A.C. and de Wildt-Eggen, J. (2007). Platelet counting in platelet concentrates with various automated hematology analyzers. Transfusion, 47: 1651-1657.
Hoffmeister, K.M., Felbinger, T.W. and Falet (2003). The clearance mechanism of chilled blood platelets. Cell, 112: 87-97.
Kahn, R.A. (1978). Biochemical changes in frozen platelets. Prog Clin Biol Res, 28: 167-180.
Klein, A., Adamik, A. and Ischke R. (1999). Changes in platelet concentrates from dogs due to storage. Part I: Platelet count and in vitro function of platelets. Beil Munch Tierarztl Wschr, 112: 243-253 (German).
Mascotti, K., McCough, J. and Burger, S.R.(2000). HPC viability measurement: trypan blue versus acridine orange and propidium iodine. Transfuion 40(6):693-696.
Selvaraj, P. (2016), Unpublished Data on Canine Clinical Apheresis, TANUVAS, Chennai.
Valeri, C.R., Feingold, H., Melaragno, A., J. (1986). Vecchione Cryopreservation of dog platelets with DMSO: Therapeutic effectiveness of cryopreserved platelets in the treatment of thrombocytopenic dogs, and the effect of platelet storage at -80˚C. Cryobiology; 23: 387-394.
Valeri, C.R., Ragno, G. and Khuri, S. (2005). Freezing human platelets with 6 percent DMSO with removal of the supernatant solution before freezing and storage at -80˚C without post thaw processing. Transfusion, 45: 1890-1898.
Downloads
Submitted
Published
Issue
Section
License
All the copy right belongs to the sponsoring Organization, Tamil Nadu Veterinary and Animal Sciences University, Chennai - 51.
How to Cite
