Antimicrobial effect of Psidium guajava, Mangifera Indica and Punica granatum extracts against bovine Mastitis pathogen

D. Silambarasan; S. Amsaveni; L. Kalaiselvi; S. Ramesh

doi:10.56093/ijvasr.v46i6.139449

Authors

D. Silambarasan Department of Veterinary Pharmacology and Toxicology, Madras Veterinary College, Chennai-600 007
S. Amsaveni Department of Veterinary Pharmacology and Toxicology, Madras Veterinary College, Chennai-600 007
L. Kalaiselvi Assistant Professor, Department of Veterinary Pharmacology and Toxicology, Madras Veterinary College, Chennai-600 007
S. Ramesh Professor and Head, Department of Veterinary Pharmacology and Toxicology, Madras Veterinary College, Chennai-600 007

https://doi.org/10.56093/ijvasr.v46i6.139449

Keywords:

Mastitis, Antibacterial activity, Psidium guajava-, Mangifera indica, Punica granatum

Abstract

The present study was carried out to assess the antibacterial activity of aqueous extracts of Psidium guajava leaves, Mangifera indica kernel and Punica granatum pericarp. Antimicrobial activity against Escherchia coli, Staphylococcus aureus and Streptococcus spp. isolated from bovine mastitis cases were tested by measuring MIC by Resazurin dye broth microdilution method. All the three plant extracts exhibited antibacterial activity against organism tested. Of the three extracts, Punica granatum extracts was found to have lower MIC of 2.5mg/ml against E. coli and Streptococcus sp compared to other two extracts. However, Mangifera indica kernel extracts was found to have good antibacterial activity against Staphylococcus aureus with a lower MIC of 0.625 mg/ml compared to other two extracts. The results of the present study confirmed the antibacterial potential of plant extracts. Further studies are required to explore the bioactive molecules which would probably become an alternative source of new and natural antibacterial agents.

Downloads

Download data is not yet available.

References

Ajariyakhajorn, K. and Samngamnim, S. (2005). Antibacterial activity of some herb extracts against bovine mastitis pathogens. The 4th Chulalongkorn University Veterinary Annual conference, Chulalongkorn University. p 102

Arima, H. and Danno, G. (2002). Isolation of antimicrobial compounds from guava (Psidium guajava L.) and their structure elucidation. Bioscience, Biotech. Biochem., 66(2): 1727- 1730.

Engels, C., Knodler, M., Zhao. Y.Y., Carle, R., Ganzle, M.G. and Schieber, A. (2009). Antimicrobial activity of gallotannins isolated from mango (Mangifera indica L.) kernels. J. Agr Food Chem., 57: 7712-7718

Kabuki, T., Nakajima, H., Arai, M., Ueda, S., Kuwabara, Y. and Dosako, S. (2000). Characterization of novel antimicrobial compounds from mango (Mangifera indica L.) kernel seeds. Food Chemistry, 71: 61-66.

Kadi, H., Moussaoui, A., Benmehdi, H., Lazouni, H.A., Benayahia, A. and Bouderba, N.N. (2011). Antibacterial activity of ethanolic and aqueous extracts of Punica granatum L. bark. Journal of Applied Pharmaceutical Science, 01 (10): 180-182

Machado, T.B., Leal, I.C.R., Amaral, A.C.F., Santos, K.R.N., Silva, M.G., Kuster, R.M. 2002. Atimicrobial ellagitannin of Punica granatum fruits. J Braz Chem Soc 13(5): 606-610.

Mirghani, M.E.S., Yosuf, F., Kabbashi, N.A., Vejayan, J. and Yosuf, Z.B.M. (2009). Antibacterial activity of mango kernel extracts. J. Applied Sci., 9: 3013-3019.

Sarker, S.D., Nahar, L., Kumarasamy, Y. (2007). Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods, 42: 321– 324.

Silva, M.A.R., Higino, J.S., Pereira, J.V., Siqueira-Junior, J.P. and Pereira, M.S.V. (2008). Antibiotic activity of the extract of Punica granatum Linn over bovine strains of Staphylococcus aureus. Rev. bras. Farmacogn, 18 (2): 209-212

Sowmiya, S., Soundarapandian, P. and Rajan, S. (2009). Bioactive Studies of Mangifera indica aganist Bacteria isolated from Urine Samples. Current Research Journal of Biological Sciences, 1(3): 139-143.