SUB-LETHAL EFFECT OF ZINC INDUCED HISTOPATHOLOGICAL ALTERATIONS IN THE GILL, LIVER AND KIDNEY OF GOLDFISH, (CARASSIUS AURATUS)

A. Subburaj; T. Francis; P. Elakkanai; N. Jayakumar

doi:10.56093/ijvasr.v44i1.147711

Authors

A. Subburaj Department of Fisheries Biology and Resource Management, Fisheries College and Research Institute, Tamil Nadu Fisheries University Thoothukudi - 628 008.
T. Francis Department of Fisheries Biology and Resource Management, Fisheries College and Research Institute, Tamil Nadu Fisheries University, Thoothukudi - 628 008.
P. Elakkanai Department of Fisheries Biology and Resource Management, Fisheries College and Research Institute, Tamil Nadu Fisheries University, Thoothukudi - 628 008.
N. Jayakumar Department of Fisheries Biology and Resource Management, Fisheries College and Research Institute, Tamil Nadu Fisheries University, Thoothukudi - 628 008.

https://doi.org/10.56093/ijvasr.v44i1.147711

Keywords:

Histopathological alteration, Goldfish, Sub-Lethal, Zinc

Abstract

The present study was carried out to find out the histopathological alterations in gill, liver and kidney of Goldfish (Carassius auratus) under sublethal concentrations of Zinc. Fishes were exposed to sub-lethal concentrations of 0.572, 1.145, 2.289, 4.578 and 9.156 mg.l -1 for 28 days which are 1/10 of their 96hr LC. The histological samples of gill, liver and kidney were collected from 14 th, 50th and 28th day and stained by haematoxylin and eosin, observed under trinocular microscope. Vacuolation, hyperplasia, epithelial lifting and lamellar fusion, shrinkage of blood vessels, secondary lamellar damage, breakdown of epithelial cells, blood congestion and necrosis were observed in gill. Rupture of nucleus, congested blood vessels, hemorrhage in liver sinuses, increased size of kupffer cell, increased pycnotic nucleus, hypertrophied hepatocytes, vacuolation, cellular necrosis, rupture of hepatocytes were observed in liver of zinc treated fish. Shrunken glomerulus, vacuolation, blood congestion, increased in space of periglomerular, increased in space of peritubular, melanomacrophages, loss of cytoplasm, fat deposition, degenerated tubules and degenerated glomerulus were seen in kidney. The above results showed that sub-lethal toxicity of Zinc alter the internal organs of goldfish at lower concentration.

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References

Abdel-Warith, A.A., Younis, E.M., Al-Asgah, N.A. and Wahbi, O.M. (2011). Effect of zinc toxicity on liver histology of Nile tilapia, Oreochromisniloticus Scientific Research and Essays, 6(17): 3760-3769.

Adhikari, S. and Ayyappan, S.(2004). Behavioral role of zinc on primary productivity, plankton and grow of a fresh water teleost. Labeo rohita (Hamilton).Aquaculture, 231(1-4): 327- 336.

Cinar, K., Aksoy, A.,Emre, Y.and Asti, N.R. (2009). The histology and histochemical aspects of gills of the flower fish, Pseudophoxinusantalyae Veterinary Research Communications, 33(5): 453-460.

Dallinger, R., Prosi, F., Senger, H. and Back, H. (1987). Contaminated food and uptake of heavy metals by fish: A Review and Proposal for Further Research. Oecologia (Berlin), 73(1): 91-98.

Deore, S.V. and Wagh, S.B.(2012). Heavy metal induced histopathological alterations in liver of Channagachua (Ham.). Journal of Experimental Sciences, 3(3): 35-38.

Donmez, H., Dursun, N.,Ozkul, Y. and Demiratas, H. (1998).Increased sister chromatin exchange in workers exposed to occupational lead and zinc. Biological Trace Elements Research, 61(1): 105-109.

Farangi, M. and Hajimoradloo, M.(2007). Clinical and histopatholoical acute toxicity signs of Ammonia in Rainbow trout (Oncorhynchusmykiss). Journal of Azadshahr Azad University, 4: 72-79.

Fathollahi, R., Khara, H., Pajand, Z.,Shenavar, A., Hallajian, A. and Moshtaghi, M.(2010). Lethal concentration detection (LC50 -96 h) of NaCl and its effects on gills of Persian sturgeon (Acipenserpersicus). Journal of Biological Sciences Azad University, Lahijan, 3 : 52-65.

Fernandez, C., Fernandes-Fernades, A., Ferreira, M. and Salgado, M.A. (2008). Oxidative stress response in gill and liver of (Liza saliens), from the Esmoriz-Paramos Coastal Lagoon, Portugal. Archives of Environmental Contamination and Toxicology, 55(2): 262-269

Garcia-Santos, S., Fontainhas-Fernandes, A. and Wilson, J.M. (2006). Cadmium tolerance in the Nile Tilapia (Oreochromisniloticus) following acute exposure: assessment of some ionoregulatory parameters. Environmental Toxicology, 21(1): 33-46.

Hallajian, A., Jourdehi, A.Y., Kazemi, R. and Shakuri, M.(2013). Acute effects of Zn (NO3)2 on gill and liver tissues of Silver Carp (Hypophthalmichthysmolitrix). Scientific Research and Essays, 8(27): 1324-1327.

Hayat, S., Javed,M.and Razzaq, S. (2007). Growth performance of metal stressed major carps viz. Catlacatla, Labeorohita and Cirrhinamrigala reared under semi-intensive culture system. Pakistan Veterianry Journal, 27(1): 8-12.

Iqbal, F., Qureshi, I.Z. and Ali, M. (2005). Histopathological changes in liver of farmed Cyprinid fish, Cyprinuscarpio, following exposure to nitrate. Pakistan Journal of Zoology, 37(4): 297-300.

Joshi, P.S.(2011). Studies on effect of zinc sulphate toxicity on detoxifying organs of fresh water fish Clariabatrachus (Linn.). Golden Research Thoughts, 1(5): 1-4.

Kamble, N.A. and Potdar, V.V. (2010). Hepatopancreatic damages in snail Bellamyabengalensis (L.) tested against lead acetate toxicity. Indian Journal of Comparative Animal Physioogy, 28(1): 7-10.

Loganathan, K., Velmurugan, B., Hongray Howrelia, J., Selvanayagam, M. and Patnaik, B.B. (2006). Zinc induced histological changes in brain and liver of Labeo rohita (Ham.). Journal of Environmental Biology, 27(1): 107-110.

Mohammad, K.A. (2003). Detection of LC and study on acute effects of Zinc on muscle tissues of gonads and liver in Common Carp (Cyprinuscarpio). M.Sc. thesis. North Branch of Tehran University.

Moshtaghi, B., Nezami, S.H., Kara, H., Pajand, Z., Shenavar, A., Hallajian, A. and Fathollahi, R.(2009). Lethal concentration detection of KMnO4 and (CuSo4.5H2O) in Acipenserpersicus fingerlings. Journal of Biological Sciences Azad University, Lahijan, 11: 67-78.

Nikam, M.T. (2012). Studies on zinc sulphate induced alternation in hepatosomatic index and renalsomatic index of air breathing fish Channapunctatus (bloch). DAV International Journal of Science,1(2): 116-118.

Paris-Palacios, S. and Biagianti-Risbourg, S.(2006). Hepatocyte nuclear structure and sub cellular distribution of copper in zebra fish Brachydaniorerio and roach Rutilusrutiluss (Teleostei, Cyprinidae) exposed to copper sulphate. Aquatic Toxicology, 77(3): 306-313.

Patra, R.C., Swarup, D., Naresh, R., Kumar, P., Shekhar, P. and Ranjan, R.(2005). Cadmium level in blood and milk from animals reared around different polluting sources in India. Bulletin of Environmental Contamination and Toxicology,76(6): 1092-1097.

Pourang, N.(1995). Heavy metal bioaccumulation in different tissues of two fish species with regards to their feeding habits and trophic levels. Environmental Monitpring and Assessment, 35(3): 207-219.

Rostami, M. and Soltani, M. (2009). Study on chronic effects of copper sulphate on some organs in Cyprinuscarpio. Journal of Veterinary Tehran University, 64: 193-198.

Rostami. M., Soltani, M. and Sasani, F. (2000). Study on histopathological effects of some heavy metals (CuSo4, ZnSo4, HgSo4 and CdSo4) on tissues of Cyprinuscarpio. Journal of Veterinary Tehran University, 55: 1-3.

Samantha, S., Mitra, K., Chandra, K., Saha, K., Bandopadhyaya, S. and Ghosh, A.(2005). Heavy metals in water of the Rivers Hoogley and Haldi and their impact on fish. Journal of Environmental Biological, 26(3) : 517-523.

Schwaiger, J., Fent, K., Stecher, H., Ferling, H. and Negele, R.D. (1996). Effects of sublethal concentrations of triphenyltinacetate on raibow trout (Oncorhynchusmykiss). Archives of Environmental Contamination and Toxicology, 30(3): 327-334.

Sprague, J.D., (1973). The ABC of Pollution Assays using fish, Biological Methods for Assessment of Water Quality, A.S.T.M. STP. 528: 6-30.

Swarup, D., Patra, R.C., Naresh, Ram, Puneet, K., PallavS. and Balagangathara thilagar, M. (2006). Deficiency of copper and cobalt in goats reared around lead zinc smelter. Small Ruminant Research, 63(3): 309-313.

Tripathi, R., (2005). Heavy metal toxicity to fresh water prawn, Macrobrachium dayanum (Crustacea-Decapoda) .Aquacultural India, 6: 57-62.

USEPA, (U.S. Environmental Protection Agency), (2003). Guidelines Establishing Test procedures for the Analysis of Pollutants, Analytical Methods for Biological Pollutants in Ambient water; Final Rule. U.S. federal Register-40 CFR Part 136(68): 139.

VanDyk, J.C., Pieterse, G.M. and VanVuren, J.H. (2007). Histological changes in the liver of Oreochromismossambicus (Cichlidae) after exposure to cadmium and zinc. Ecotoxicology and Environmental Safety, 66(3): 432-440.

Vinodhini, R. and Narayanan, M. (2008). Bioaccumulation of heavy metals in organs of fresh water fish Cyprinuscarpio (Common carp). International Journal of Environment Science and Technology,5(2): 179-182.

Watson, C.F. and Benson, W.H. (1987). Comparative activity of gill ATPase in three freshwater teleosts exposed to cadmium. Ecotoxicology and Environmental Safety,14(3): 252-259.

Wong, C.K. and Wong, M.H. (2000). Morphological and biochemical changes in the gills of tilapia (Oreochromismossambicus) to ambient cadmium exposure. Aquat. Toxicol., 48(4): 517-527.

Woodling, J.D., Brinkman, S.F. and Horn, B.J. (2001). Non uniform accumulation of cadmium and copper in kidneys of wild brown trout Salmotrutta population. Arch. Environ. Contam. Toxicol., 40(3): 381-385.