MOLECULAR STUDY OF GROWTH HORMONE AND GROWTH HORMONE RECEPTOR GENES IN MEHSANA BUFFALO
DOI:
https://doi.org/10.56093/ijvasr.v43i4.153127Keywords:
Buffalo, Growth hormone, Growth hormone receptor, PCR-RFLPAbstract
The study was conducted to find out polymorphism at various growth hormone (bGH) and growth hormone receptor loci (bGHR) by using PCRRFLP technique. Fifty two blood samples of Mehsana buffalo were collected from Livestock Research Station, Sardarkrushinagar Agricultural University, Gujarat. The DNA samples were subjected to PCR amplification using bGH and bGHR specific primers. The PCR products of GH1 (436 bp), GH2 (891bp), GH3 (441bp) and GHR1 (approx 640 bp) loci were digested with Alu 1, Msp 1, Hae III and Mae II restriction enzymes respectively and only AA, CC, FF and RR genotypes were respectively found in Mehsana buffalo. All the bGH and bGHR loci studied were monomorphic.
Downloads
References
Bauman, D.E. and McCutcheon, S.N. (1986). The effects of growth hormone and prolactin on metabolism. In: Control of digestion and metabolism in ruminants (Ed. L. P.Miligan, W. I. Groven and A. Dobson). Prentice-Hall, England Cliffs.N.J. p. 436.
Biswas, T.K., Bhattacharya, T.K., Narayan, A.D., Badola, S., Kumar, P. and Sharma, A. (2003). Growth hormone gene polymorphism and its effect on birth weight in cattle and buffalo. Asian Australasian Journal of Animal Sciences 16: 494-497.
Ge, W., Davis, M. E., Hines, H. C. and Irvin, K. M. (2000). Rapid communication: Single nucleotide polymorphisms detected in exon 10 of the bovine growth hormone receptor gene. Journal of Animal Science, 78: 2229–2230.
John, S.W.M., Weitzner, G., Rozen, R. and Scriver, C.R. (1991). A rapid procedure for extracting genomic DNA from leucocytes. Nucleic Acid Research 19: 408.
Lucy, M.C., Hauser, S.D., Eppard, P.J., Krivi, G. G., Clark, J. H., Bauman, D. E. and Collier, R. J. (1991). Genetic polymorphism within the bovine somatotropin (bST) gene detected by polymerase chain reaction and endonuclease digestion. Journal of Dairy Science 74: 284.
Mitra, A., Schlee, P., Balakrishnan, C.R. and Pirchner, F. (1995). Polymorphisms at growth hormone and prolactin loci in Indian cattle and buffalo. Journal of Animal Breeding and Genetics, 112: 71-74.
Pawar, R.S. (2005). Growth hormone and growth hormone receptor gene polymorphism in relation with milk production in dairy cattle. M.V.Sc thesis submitted to Sardarkrishinagar Dantiwada Agricultural University, Sardarkrishinagar.
Unanian, M.M., DeNise, S.K., Zhang, H.M. and Ax, R.L. (1994). Rapid communication: polymerase chain reaction-restriction fragment length polymorphism in the bovine growth hormone gene. Journal of Animal Science 72 : 2203.
Zhang, H.M., Brown, D.R., DeNise, S.K. and Ax, R.L. (1992). Nucleotide sequence determination of a bovine somatotropin allele. Animal Genetics, 23: 578.
Zhang, H.M., Brown, D.R., DeNise, S.K. and Ax, R.L. (1993). Rapid communication: Polymerase chain reaction-restriction fragment length polymorphism analysis of the bovine somatotropin gene. Journal of Animal Science, 71: 2276.
Downloads
Published
Issue
Section
License
All the copy right belongs to the sponsoring Organization, Tamil Nadu Veterinary and Animal Sciences University, Chennai - 51.