EFFECT OF POMEGRANATE (Punica granatum) JUICE ON PLASMA  NITRIC OXIDE LEVELS AND ANTIOXIDANT STATUS IN ISOLATION STRESS  AND HEAT STRESS INDUCED RATS

V. Beckseeba Lavanya; A. Thangavel; V. Leela; S. Selvasubramanian

doi:10.56093/ijvasr.v9i1.154498

Authors

V. Beckseeba Lavanya M.V.Sc. Student, Tamil Nadu Veterinary and Animal Sciences University, Chennai – 600 051
A. Thangavel Professor and Head, Department of Veterinary Physiology, Madras Veterinary College, Chennai – 600 007
V. Leela Professor, Department of Veterinary Physiology, Madras Veterinary College, Chennai – 600 007
S. Selvasubramanian Professor, Department of Pharmacology and Toxicology, Madras Veterinary College, Chennai – 600 007.

https://doi.org/10.56093/ijvasr.v9i1.154498

Keywords:

Pomegranate juice (PJ), Isolation stress and heat stress induced – Nitric oxide (NO) - SOD, CAT and GSH-Px, lipid peroxidation – Rats

Abstract

This study was undertaken to assess the effect of pomegranate (Punica granatum) juice (PJ) supplementation on plasma nitric oxide (NO) levels and antioxidant status in isolation stress induced and heat stress induced rats. Thirty adult male Wistar albino rats of 140-150 g body weight were randomly divided into five groups of six each viz., control, isolated stress induced, isolation stress induced supplemented with PJ, heat stress induced and heat stress induced supplemented with PJ. Blood samples were collected at weekly interval during four weeks of the experimental period, plasma samples were separated and stored at -20⁰C for the assay of nitric oxide (NO) levels, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) activities and lipid peroxidation levels. PJ supplementation to isolated stress induced and heat stress induced rats significantly (Pd” 0.05) increased plasma nitric oxide (NO) levels, SOD, CAT and GSH-Px activities, whereas plasma lipid peroxidation level was significantly lowered (Pd” 0.05) in comparison with respective stress induced models and control groups.

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