NUCLEOLUS  ORGANIZER  REGION (NOR)  BANDING  IN MAHABUBNAGAR  LOCAL GOATS

P. Uma Devi; B. Ramesh Gupta; K. Sakunthala Devi

doi:10.56093/ijvasr.v9i3.155313

Authors

P. Uma Devi Veterinary Assistant Surgeon, Veterinary Dispensary, Kurnool - 518 510
B. Ramesh Gupta Department of Animal Genetics and Breeding, College of Veterinary Science, Rajendranagar, Hyderabad-500 030
K. Sakunthala Devi Department of Animal Genetics and Breeding, College of Veterinary Science, Rajendranagar, Hyderabad-500 030

https://doi.org/10.56093/ijvasr.v9i3.155313

Keywords:

nucleolus, organizer, region, banding, goats

Abstract

Nucleolus organizer regions (NORs) on the chromosomes of Mahabubnagar local goats were studied using shot-term lymphocyte culture technique. The ammoniacal silver staining procedure was followed with minor modifications . The chromosomes were stained with Silver-Giemsa staining technique using gelatin colloidal developer. The frequency distribution of number of metaphases containing the Ag-NOR bands were tested for significance by Chi-square test. A total of 213 NORs were detected in 42 metaphases. The mean number of NORs per metaphase was 5.071, with a range from 4.72 to 6.17, while the number of NORs per metaphase ranged from 1 to 13. Frequency of the metaphases reduced as the number of NORs per metaphase increased. The Chi-square test of significance has revealed that the observed frequencies departed significantly from the expected frequencies (P<0.05). The highest frequency of metaphases (21.43%) examined had one NOR, while the lowest frequency (2.38%) had 9 or 11 NORs. The chromosome morphology, number and morphometric measurements and NORs in Mahabubnagar local goats were similar to those of the descript breeds of goats in India.

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