VALIDATION OF SIMPLE ISOCRATIC HPLC ASSAY FOR THE DETERMINATION OF CHLORTETRACYCLINE CONCENTRATION IN PLASMA OF CHICKEN

M. Nivetha; S. Ramesh; L. Kalaiselvi; R. Gokul; T. Ramasamy

doi:10.56093/ijvasr.v53i5.162203

Authors

M. Nivetha M.V.Sc. Student, Dept. of Veterinary Pharmacology and Toxicology, Madras Veterinary College, Chennai
S. Ramesh Professor and Head, Dept. of Veterinary Pharmacology and Toxicology, MVC, Chennai
L. Kalaiselvi Assistant Professor, Dept. of Veterinary Pharmacology and Toxicology, MVC, Chennai
R. Gokul M.V.Sc. Student, Dept. of Veterinary Pharmacology and Toxicology, Madras Veterinary College, Chennai
T. Ramasamy Assistant Professor, Dept. of Veterinary Pharmacology and Toxicology, MVC, Chennai

https://doi.org/10.56093/ijvasr.v53i5.162203

Keywords:

Chlortetracycline, HPLC, chicken plasma, pharmacokinetics

Abstract

Chlortetracycline (CTC) is a broad-spectrum antibiotic belonging to the tetracycline group of drugs. It is commonly used in the poultry industry for the treatment of bacterial infections and is also used as a growth promoter. This study was aimed at validating the sensitive HPLC method for the assay of CTC in chicken plasma. The method consisted of isocratic elution with separation using a C_]8 column. The mobile phase used in the study was aqueous oxalic acid (0.03M), acetonitrile and methanol in the ratio of 60:30:10. The HPLC conditions included a flow rate of Iml/min with UVdetection at 375nm. CTC in chicken plasma was extracted with Mcllvaine buffer followed by dilution with water in the ratio of 1:1 andfiltered using 0.22p filters. The peak of chlortetracycline was noticed at 5-6 minis. The method was linear from 0.05mg/ml to 10mg/ml. The recovery percentage from plasma was recorded as 110%. Thus, from this study it is inferred that this method is optimal for the assay of CTC in plasma for pharmacokinetic studies.

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