MOLECULAR DIAGNOSIS AND RISK FACTORS ANALYSIS OF HEPATOZOON CANIS INFECTION IN DOGS

Kopparthi Jalajakshi; Chennuru Sridevi; Vukka Chengalva Rayulu

doi:10.56093/ijvasr.v55i3.179312

Authors

Kopparthi Jalajakshi Assistant Professor, Department of Veterinary Parasitology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati - 517 502. Andhra Pradesh
Chennuru Sridevi Professor, Department of Veterinary Parasitology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati - 517 502. Andhra Pradesh
Vukka Chengalva Rayulu Professor and Controller of Examinations, Department of Veterinary Parasitology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati - 517 502. Andhra Pradesh

https://doi.org/10.56093/ijvasr.v55i3.179312

Keywords:

Risk factors analysis, microscopy, polymerase chain reaction, H.canis and sequencing

Abstract

In this study we analysed risk factors and molecular diagnosis of Hepatozoonosis in the naturally infected canines of Andhra Pradesh, using thin blood smears and polymerase chain reaction. Four hundred and forty-two dog blood samples were collected and examined by microscopy and conventional PCR. Microscopic examination detected Hepatozoon canis organisms in four dogs with 0.90% case prevalence (4/442)while,18S rRNA gene conventional PCR assay detected H. canis in twelve dogs with 2.71% case prevalence (12/442). The partial 18S rRNA gene of H.canis amplified from conventional PCR, underwent DNA sequencing. The 18S rRNA gene sequences were then analysed using BLAST, confirming their identity as H. canis through comparison with existing sequences in the GenBank database.Under risk factors analysis the living condition of the dogs (stray dogs) and tick infestation were identified as major risk factors causing higher prevalence rates in canines of Andhra Pradesh.

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