Estimation of Thyroid profile in cats through Radio Immunoassay

Purvi M. Naik; Chandrakant Narayanrao Galdhar; A. R. Dadke; R. V. Gaikwad; K. V. Garud; S.D. Ingole; S.D. Moregaonkar

doi:10.56093/ijvm.v46i1.174261

Authors

Purvi M. Naik Department of Veterinary Clinical Medicine, Ethics and Jurisprudence, Mumbai Veterinary College, Maharashtra Animal and Fishery Sciences University, Nagpur Parel, Mumbai-12
Chandrakant Narayanrao Galdhar Department of Veterinary Nuclear Medicine including Radio Isotope Laboratory, Mumbai Veterinary College, Parel-Mumbai-12
A. R. Dadke Department of Veterinary Clinical Medicine, Ethics and Jurisprudence, Mumbai Veterinary College, Maharashtra Animal and Fishery Sciences University, Nagpur Parel, Mumbai-12
R. V. Gaikwad Department of Veterinary Clinical Medicine, Ethics and Jurisprudence, Mumbai Veterinary College, Maharashtra Animal and Fishery Sciences University, Nagpur Parel, Mumbai-12
K. V. Garud Department of Veterinary Clinical Medicine, Ethics and Jurisprudence, Mumbai Veterinary College, Maharashtra Animal and Fishery Sciences University, Nagpur Parel, Mumbai-12
S.D. Ingole Department of Veterinary Clinical Medicine, Ethics and Jurisprudence, Mumbai Veterinary College, Maharashtra Animal and Fishery Sciences University, Nagpur Parel, Mumbai-12
S.D. Moregaonkar Department of Veterinary Clinical Medicine, Ethics and Jurisprudence, Mumbai Veterinary College, Maharashtra Animal and Fishery Sciences University, Nagpur Parel, Mumbai-12

https://doi.org/10.56093/ijvm.v46i1.174261

Keywords:

Feline thyroid function, Radioimmunoassay (RIA); Triiodothyronine (TT3); Thyroxine (TT4) and Free Thyroxine (fT4).

Abstract

Radioimmunoassay (RIA) is a highly sensitive and specific in vitro technique for quantifying hormones and other small antigens in biological fluids. The present study was undertaken to establish reference values for total Triiodothyronine (TT3), total Thyroxine (TT4), and free Thyroxine (fT4) in healthy cats. A total of thirty-one (31) healthy cats were ethically enrolled for the establishment of the thyroid hormone reference range. Serum concentrations of TT3, TT4, and fT4 were measured using RIA. The estimation of TT3 was carried out using the procedure recommended by the manufacturers. However, TT4 and fT4 were estimated by doubling the serum and standard volumes and halving the final results. The results of the present study recorded mean serum TT3, TT4, and fT4 values in healthy cats as 0.69 ± 0.13 nmol/l, 28.29 ± 3.78 nmol/l and 31.41 ± 2.88 pmol/l respectively. The reference interval (25^th to 75^th percentile) for TT3, TT4, and fT4 was found to be 0.31 – 0.84 nmol/l, 13.84 – 34.75 nmol/l and 19.31 – 37.32 pmol/l respectively. The median for TT3, TT4, and fT4 was found to be 0.38 nmol/l, 19.95 nmol/l and 33.46 pmol/l respectively.

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Author Biography

Chandrakant Narayanrao Galdhar, Department of Veterinary Nuclear Medicine including Radio Isotope Laboratory, Mumbai Veterinary College, Parel-Mumbai-12

Professor and Radiological Safety Officer,

References

Anderson, J.H. and Brown, R.E. 1979. Serum thyroxine (T4) and triiodothyronine (T3) uptake values in normal adult cats as determined by radioimmunoassay. Am. J. Vet. Res.. 40(10): 1493–1494.

Crowe, A. 2004. Congenital hypothyroidism in a cat. Canadian Vet. J, 45: 168–170.

Dadke, A.R., Galdhar, C.N., Gaikwad, R.V., and Kadam, D.P. 2018. Studies on biochemical alterations in hypothyroid dogs. Indian J. Vet.Med., 38: 35–38.

Galdhar, C., Chandra, S., Dadke, A., Gaikwad, R. and Sarode, A. 2021. Metabolic and hormonal changes in water buffaloes during postparturient peak lactation. Buffalo Bulletin. 40: 565–570.

Galdhar, C.N., Gaikwad, R.V., Masare, P.S., Garud, K.V., Vaidya, S., Bhojne, G.R., et al. and Parkhe, A.G. (2024). Triiodothyronine (TT3), total thyroxine (TT4) and free thyroxine (FT4) reference range in healthy dogs by radioimmunoassay (RIA). Indian Journal of Animal Research. 58(1): 46–50.

Galdhar, C.N., Vaidya, S.M. and Gaikwad R.V. 2022. Serum Triiodothyronine (T) And Thyroxine (T) Levels in Healthy Goats. Indian J. Small Ruminants, 28(2): 403-404.

Galdhar C.N. Shubham Vyas, R.V. Gaikwad, J.R. Kawade, and P.M. Sapkal. 2024. Studies on the Assessment of Hair Cortisol Concentration in Dogs by Radioimmunoassay (RIA). Indian J. Anim. Res., doi: 10.18805/IJAR.B-5221.Online: 10-05-2024

Jayabhaye, R.M., Galdhar, C.N., Dadke, A.R., Gaikwad, R.V., Banik, S. and Rajkhowa, S. 2021. Radio immune assay (RIA) enabled thyroid profile (TT3, TT4 and fT4) in pigs. Indian J. Vet. Med., 41: 9–15.

Mooney, C.T. 2010. Hyperthyroidism. In: Ettinger, S. and Feldman, E. (eds.), Textbook of Veterinary Internal Medicine: Diseases of the Dog and Cat. W.B. Saunders, Philadelphia. pp. 1761–1779.

Peterson, M.E., Guterl, J., Nichols, R. and Rishniw, M. 2015. Evaluation of serum thyroid stimulating hormone concentration as a diagnostic test for hyperthyroidism in cats. Journal of Veterinary Internal Medicine. 29: 1169–1170.

Peterson, M.E., Kintzer, P.P. and Cavanagh, P.G. 1983. Feline hyperthyroidism: pretreatment clinical and laboratory evaluation of 131 cases. J. Vet. Internal Med.,183: 103–110.

Peterson, M.E., Kintzer, P.P., Fox, P.R., Ferguson, D.C. and Johnson, G.F. 1981. Feline hyperthyroidism: pretreatment clinical and laboratory evaluation of 131 cases. J. Am. Vet. Med. Assoc., 183: 103–110.

Peterson, M.E., Melián, C. and Nichols, R. 2001. Measurement of serum concentrations of free thyroxine, total thyroxine and total triiodothyronine in cats with hyperthyroidism and cats with non-thyroidal disease. J. Am. Vet. Med. Assoc., 218(4): 529–536.

Reap, M., Cass, C. and Hightower, D. (1978). Thyroxine and triiodothyronine levels in ten species of animals. Southwest Veterinary. 31: 31.

Reimers, T.J., Cowan, R.G., Davidson, H.P. and Colby, E.D. (1982). Validation of radioimmunoassays for triiodothyronine, thyroxine and hydrocortisone (cortisol) in canine, feline and equine sera. Am. J. Vet.Res.,42(11): 2016–2021.

Salutgi, P., Galdhar, C., Sonigra, R., Natu, K., Mumbarkar, N., Mathkar, S., Dalvie, A. and Gaikwad, R. (2023). Radio immune assay (RIA) enabled total triiodothyronine (TT3) and total thyroxine (TT4) in canine trypanosomiasis: first case report from Maharashtra (India). Iranian J. Parasitol., 18: 107–112.

Snedecor, G. W., and Cochran, W. G. 2004. Statistical methods, 8thEdn. Ames: Iowa State Univ. Press Iowa, 54, 71-82.

Thoday, K.L., Setht, J. and Elton, R.A. 1984. Radioimmunoassay of serum total thyroxine and triiodothyronine in healthy cats: assay methodology and effects of age, sex, breed, heredity and environment. J. Small Anim. Pract., 25: 451–412.

Yalow, R.S. and Berson, S.A. (1960). Immunoassay of endogenous plasma insulin in man. J. Clinical Investigation. 39: 1157–1175.