Presence of Multiple Drug Resistant Enterococcus faecalis with Virulence Traits in Household Dahi
143 / 47
Authors
-
S Bhagya
Department of Dairy Microbiology, College of Dairy Science and Technology, Pookode, Wayanad,Kerala,673576
-
Archana Chandran
Department of Dairy Microbiology, College of Dairy Science and Technology, Pookode, Wayanad,Kerala,673576
-
R.L Rathish
Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Pookode, Wayanad,673576
-
M.P Rahila
Department of Dairy Chemistry, College of Dairy Science and Technology, Pookode, Wayanad,Kerala,673576
-
E.C Athulya
Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Pookode, Wayanad,673576
Keywords:
Enterococcus faecalis, fermented milk, virulence, antimicrobial resistance, household foodsAbstract
Dahi is a traditional fermented milk made using undefined starter cultures. An integrated approach based on phenotypic and genotypic methods were conducted to investigate the virulence traits and multiple drug resistance of Enterococcus faecalis strains isolated from dahi. Eight strains of E.faecalis were isolated from house hold dahi. Multiple drug resistance towards Ceftriaxone and Vancomycin was exhibited by the strain E.faecalis ADMH 19. The gene coding for gel E and asa1 were detected and no gene coding for clyA was observed. The presence of Enterococci with multiple drug resistance and virulence factors in homemade foods can be a potential risk to the consumers.
Downloads
References
Almeida L. M., Lebreton, F., Gaca, A., Bispo, P.M., Saavedra, J.T., Calumby, R.N., and Gilmore, M. S.. Transferable resistance gene optrA in Enterococcus faecalis from Swine in Brazil. Antimicrobial agents and chemotherapy. 64(6): 1-11.
Archana, C., Beena, A.K., Bhagya, S., Rathish, R.L, and Rahila, M.P. (2022) Probiotic characterisation of Enterococcus faecalis strain isolated from a household dahi sample of Wayanad district, Kerala J. Vet. Anim. Sci.. 53(1): 70-78.
Barrow, G.I., and Feltham, R.K.A. (1993) Cowan and Steel’s manual for the identification of medical bacteria. Cambridge University Press, Cambridge, UK. 331 pp.
Coque, T.M., Patterson, J.E., Steckelberg, J.M., and Murray, B.E. (1995) Incidence of hemolysin, gelatinase, and aggregation substance among enterococci isolated from patients with endocarditis and other infections and from feces of hospitalized and community-based persons. Journal of Infectious Diseases. 171(5):1223-1229.
Del Papa, M.F., Hancock, L.E., Thomas, V.C., and Perego, M. (2007) Full activation of Enterococcus faecalis gelatinase by a C-terminal proteolytic cleavage. Journal of bacteriology. 189(24): 8835-8843.
Del Re, B., Sgorbati, B., Miglioli, M., and Palenzona. (2000) Adhesion, autoaggregation and hydrophobicity of 13 strains of Bifidobacterium longum. Lett. Appl. Microbiol. 31: 438-442.
Economou, V., Sakkas, H., Delis, G., and Gousia, P. (2017) Antibiotic resistance in Enterococcus spp. Friend or foe?. Foodborne Pathogens and Antibiotic resistance, ed. OM Singh (Hoboken, NJ: John Wiley & Sons).
Galli, D., Lottspeich, F., and Wirth, R. (1990) Sequence analysis of Enterococcus faecalis aggregation substance encoded by the sex pheromone plasmid pAD1. Molecular microbiology. 4(6): 895-904.
Golob, M., Pate, M., Kušar, D., Dermota, U., Avberšek, J., Papić, B., and Zdovc, I. (2019) Antimicrobial resistance and virulence genes in Enterococcus faecium and Enterococcus faecalis from humans and retail red meat. BioMed research international.
Guzman, C.A., Pruzzo, C., LiPira, G., and Calegari, L. (1989) Role of adherence in pathogenesis of Enterococcus faecalis urinary tract infection and endocarditis. Infection and immunity. 57(6): 1834-1838.
Haas, W., Shepard, B.D., and Gilmore, M.S. (2002) Two-component regulator of Enterococcus faecalis cytolysin responds to quorum-sensing autoinduction. Nature. 415(6867): 84-87.
Harun-ur-Rashid, M., Togo, K., Ueda, M., and Miyamoto, T. (2007) Probiotic characteristics of lactic acid bacteria isolated from traditional fermented milk “Dahi” in Bangladesh. Pakistan J. Nutr. 6: 647-652.
Muñoz-Atienza, E., Gómez-Sala, B., Araújo, C., Campanero, C., Del Campo, R., Hernández, P.E., and Cintas, L.M. (2013) Antimicrobial activity, antibiotic susceptibility and virulence factors of lactic acid bacteria of aquatic origin intended for use as probiotics in aquaculture. BMC microbiology. 13(1): 1-22.
Nami, Y., Abdullah, N., Haghshenas, B., Radiah, D., Rosli, R., and Yari Khosroushahi, A. (2014) A newly isolated probiotic Enterococcus faecalis strain from vagina microbiota enhances apoptosis of human cancer cells. Journal of applied microbiology. 117(2): 498-508.`
Shangpliang, H.N.J., Sharma, S., Rai, R., and Tamang, J.P. (2017) Some technological properties of lactic acid bacteria isolated from Dahi and Datshi, naturally fermented milk products of Bhutan. Front.Microbiol. 8: 116.
Su, Y.A., Sulavik, M.C., He. Ping., Makinen, K.K., Makinen, P.L., Fiedler, S., and Clewell, D.B. (1991) Nucleotide sequence of the gelatinase gene (gelE) from Enterococcus faecalis subsp. liquefaciens. Infection and immunity. 59(1) : 415-420.
Waters, C.M., Antiporta, M.H., Murray, B.E., and Dunny, G.M. (2003) Role of the Enterococcus faecalis GelE protease in determination of cellular chain length, supernatant pheromone levels, and degradation of fibrin and misfolded surface proteins. Journal of bacteriology. 185(12): 3613-3623.
