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Evaluation of Interaction between Oxytetracycline and Aflatoxin B1-Induced Cytotoxicity


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Authors

  • T. Jagadeesh Department of Veterinary Pharmacology and Toxicology, Madras Veterinary College, Chennai-600007, Tamil Nadu, India
  • M. Parthiban Department of Animal Biotechnology, Madras Veterinary College, Chennai-600007, Tamil Nadu, India
  • P. Raja Department of Animal Biotechnology, Madras Veterinary College, Chennai-600007, Tamil Nadu, India.
  • Ghadevaru Sarathchandra Pharmacovigilance Laboratory for Animal Feed and Food Safety, Tamil Nadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, Chennai-600051, Tamil Nadu, India
  • S. Vairamuthu Centralized Clinical Laboratory, Madras Veterinary College, Chennai-600007, Tamil Nadu, India

https://doi.org/10.62757/3csd5q50

Keywords:

Aflatoxin B1, Oxytetracycline, Molecular docking, In vitro study, Vero cell line

Abstract

To mitigate the toxicity of aflatoxin B1 (AFB1) in animals, additional therapeutic agents are needed due to the limitations of mycotoxin binders. AFB1-induced cytotoxicity is mediated by mitogen activated protein kinase pathways (MAPK). It has been reported that oxytetracycline (OTC) and its analogues have been shown to inhibit the activity of the MAPK pathway. Therefore, it is important to draw attention to this context to know the effect of OTC on AFB1-induced cytotoxicity. The results from molecular docking revealed that OTC interacted more positively with the MAPK1 protein. In vitro studies revealed that the 50μg/mL concentration of OTC effectively protects the cytotoxicity induced by AFB1 using MTT and Trypan blue techniques.

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Submitted

2023-08-24

Published

2023-08-24 — Updated on 2024-04-15

Versions

Issue

Vol. 100 No. 8 (2023): August, 2023

Section

Articles

How to Cite

T. Jagadeesh, M. Parthiban, P. Raja, Ghadevaru Sarathchandra, & S. Vairamuthu. (2024). Evaluation of Interaction between Oxytetracycline and Aflatoxin B1-Induced Cytotoxicity. The Indian Veterinary Journal, 100(8). https://doi.org/10.62757/3csd5q50 (Original work published 2023)