Anticancerous effect of Annona squamosa (Custard apple) seed Extract in Breast cancer-An In-Silico Approach

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  • B. Behera TANUVAS, Madhavaram Milk Colony, Chennai – 600051
  • N. Pazhanivel TANUVAS, Madhavaram Milk Colony, Chennai – 600051
  • S. Vairamuthu TANUVAS, Madhavaram Milk Colony, Chennai – 600051
  • S. Sureshkannan TANUVAS, Madhavaram Milk Colony, Chennai – 600051
  • T.M.A. Senthil Kumar TANUVAS, Madhavaram Milk Colony, Chennai – 600051
  • P. Jalantha TANUVAS, Madhavaram Milk Colony, Chennai – 600051
  • M.R. Srinivasan TANUVAS, Madhavaram Milk Colony, Chennai – 600051
  • Ganne Venkata Sudhakar Rao TANUVAS, Madhavaram Milk Colony, Chennai – 600051


Anticancerous, Breast Cancer, Custard apple, In-silico


Cancer is a serious health problem affecting both developing and developed countries despite considerable efforts to improve therapy. Breast cancer (BC) is one of the commonest cancer among women globally. Phytochemical and pharmacological studies on A. squamosa seeds have shown that the major bioactive compounds are annonaceous acetogenins. Custard apple seeds are well known for their interesting pharmacological activities, especially for antitumor activity. The primary purpose of molecular docking is to predict the affinity of a drug candidate to bind with the protein and form the most stable complex. The molecular docking method has several advantages, such as reducing cost and time in drug design. IMPHY014813 ligand has more interaction with Estrogen receptors having a docking score of 164.195 and an interaction energy of 42.4072. IMPHY001193 ligand interaction with Progesterone receptor showed more doc score i.e. 176.752 and interaction energy 46.7546. IMPHY003104 compound showed interaction with Caspase3 with a doc score of 75.7933 and interaction energy of 22.1722. IMPHY000157 compound showed interaction with Nf-kb p65 and hada doc score of 124.101 and interaction energy of 63.6814.


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Bermejo A., Figadere B., Zafra-Polo M.C., Barrachina I., Estornell E., and Cortes, D. (2005) Acetogenins from Annonaceae: recent progress in isolation, synthesis, and mechanisms of action. Natural Products Reports,22: 269–303.

Besli N., Yenmis G, Tuncdemir M., Sarac E.Y., Dooan S., Solakoolu S., and Sultuybek, G.K. (2020) Metformin suppresses the proliferation and invasion through Nf-kB and MMPs in MCF-7 cell line. Turkish Journal of Biochemistry. 45(3): 295-304.

Bray F., Ferlay J., Soerjomataram I., Siegel R.L., Torre L.A., and Jemal A. (2018) Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer Journal for Clinicians,68: 394-424.

Chen J.W., Chen Y., and Li X. (2011) Beneficial aspects of custard apple (Annona squamosa L.) seeds. In: Preedy V.R., Watson R.R., Patel V.B. (Eds.), Nuts & Seeds in Health and Disease Prevention. Elsevier Academic Press, London, Burlington, San Diego, pp. 439–445.

Damayanti D.S., Utomo D.H., and Kusuma C. (2017). Revealing the potency of Annona muricata leaves extract as FOXO1 inhibitor for diabetes mellitus treatment through computational study. In silico pharmacology, 5 (1): 3-7

Devarajan E., Sahin A.A., Chen J.S., Krishnamurthy R.R., Aggarwal N., Brun A.M., Sapino A., Zhang F., Sharma D., Yang X.H., and Tora A.D. (2002) Down-regulation of Caspase 3 in breast cancer: a possible mechanism for chemoresistance. Oncogene, 21(57): 8843-8851

Fitriah A., Holil K., Syarifah U., Fitriyah U., and Utomo D.H. (2018) In silico approach for revealing anti-breast cancer and estrogen receptor alpha inhibitory activity of Artocarpus altilis In AIP Conference Proceedings,1: 1-7

Gul H.F., Ilhan N., Ozercan I.H., and Kuloglu T. (2021) The combined effect of pomegranate extract and tangerine on the DMBA-induced breast cancer model, Journal of Nutrition and Biochemistry, 89: 108566.

Herrmann K. (2019) Refinement on the way towards replacement: Are we doing what we can? In Herrman K., Jane K., editors. Animal Experimentation: Working towards a Paradigm Change. Boston: Brill; pp. 1–64.

India State-Level Disease Burden Initiative Collaborators. (2017) Nations within a nation: variations in epidemiological transition across the states of India, 1990-2016 in the global burden of disease study, Lancet,390: 2437-60.

Kucinska M., Giron M.D., Piotrowska H., Lisiak N., Granig W.H., Lopez-Jaramillo F.J., Salto R., Murias M., and Erker T. (2016) Novel promising estrogenic receptor modulators: cytotoxic and estrogenic activity of benzanilides and dithiobenzanilides. PLoS One,11(1): e0145615.

Lange C.A., and Yee, D. (2008) Progesterone and breast cancer. Women’s Health4: 151-162.

Le D., Chen K., Husain S., Marathe A. and Haq M. (2018) Molecular genetics of cancer. International Journal of Human and Health Sciences, 2: 199-208.

Li Y. F., & Fu, L.W. (2004) Antitumoral effects of annonaceous acetogenins. Chinese Pharmacology Bulletin, 20(3): 245-247.

Liaw C.C., Wu T.Y., Chang F.R., and Wu Y.C. (2010) Historic perspectives on Annonaceous acetogenins from the chemical bench to preclinical trials. Planta Medica, 76: 1390–1404.

Lipinski C.A., Lombardo F., Dominy B.W., and Feeney, P.J. (1997) Experimental and computational approaches to estimate solubility and permeability in drug discovery and development settings. Advanced Drug Delivery Reviews. 23(1-3): 3-25.

Liu H.X., Huang G.R., & Zhang H.M. (2007). Annonaceous acetogenin mimics bearing a terminal lactam and their cytotoxicity against cancer cells. Bioorganic & Medicinal Chemistry Letters, 17: 3426-3430.

Mirkin S., and Pickar J.H. (2015) Selective estrogen receptor modulators (SERMs): a review of clinical data. Maturitas80(1):52–57.

Platet N., Cathiard A.M., Gleizes M., and Garcia M. (2004) Estrogens and their receptors in breast cancer progression: a dual role in cancer proliferation and invasion, Cancer Rev. Oncology and Hematology. 51: 55–67.

Siegel R.L., Miller K.D., and Jemal A. (2018) Cancer statistics. CA Cancer Journal for Clinicians, 68: 7-30.

Sung H., Ferlay J., Siegel R.L., Laversanne M., Soerjomataram I., Jemal A., and Bray F. (2021) Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA Cancer Journal for Clinicians, 71: 209-249.

Wakui S., Yokoo K., Takahashi H., Muto T., Suzuki Y., Kanai Y., Hano H., Furusato M., and Endou H. (2005) CYP1 and AHR expression in 7,12-dimethylbenz(a) anthracene-induced mammary carcinoma of rats prenatally exposed to 3,3,4,4,5- pentachlorobiphenyl, Toxicology211: 231–241.

Workman P., Aboagye E.O., Balkwill F., Balmain A., Bruder G., Chaplin D.J., et al. (2010) Guidelines for the welfare and use of animals in cancer research. British Journal of Cancer, 102(11): 1555–1577. https://





How to Cite

B. Behera, N. Pazhanivel, S. Vairamuthu, S. Sureshkannan, T.M.A. Senthil Kumar, P. Jalantha, M.R. Srinivasan, & Ganne Venkata Sudhakar Rao. (2023). Anticancerous effect of Annona squamosa (Custard apple) seed Extract in Breast cancer-An In-Silico Approach. The Indian Veterinary Journal, 100(12), 37-43.