Molecular detection of Dirofilaria immitis in dogs
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Authors
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Wasan A Alobaidii
Department of microbiology, college of veterinary medicine, Mosul University, Iraq
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Shahad Abbas Ali
Department of parasitology, college of veterinary medicine, Kirkuk University, Iraq
Keywords:
Dirofilariaimmitis , Dogs,Polymerase Chain Reaction,MosulAbstract
There are different species of nematodes which affect dogs health but the Dirofilariaimmitisis the dangerous one , in this study2 ml of blood from one hundred four of samples, from dogs different breeds,breeding managements and age range from less than 6 months to more than 4 years old , then DNA extracted from this blood , PCR technique using a Primer designed as ( 5-AGTGTAGGTCAGCCCTGAGTTA-3 ) forward and ( 5-ACAGGCACTGACAATACCAAT-3 ) was used to amplify 203 bp in mitochondrial oxidase (COI), COI gene amplification results showed 203 bp of band for 15 blood samples.not Anyof the negative samples for D.immitis give amplification of the DNA molecules, 15 samples from 104 animalsshowed positive using PCR (14.4%) ,high prevelance of infection in dogs 2-4years old (40%) , while the animals less than one year old not recorded any infection rates, Outdoor animals showed high prevalence of infection with D.immitis when compare with indoor once
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