Isolation and Identification of Brucella Melitensis in Diseased Nile Tilapia, Oreochromis Niloticus from a Commercial Fish Farm in Tamil Nadu, India


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Authors

  • A. Uma State Referral Laboratory for Aquatic Animal Health, Tamil Nadu Dr.J.Jayalalithaa Fisheries University-Madhavaram campus, Chennai, Tamil Nadu-600 051, India
  • G. Rebecca State Referral Laboratory for Aquatic Animal Health, Tamil Nadu Dr.J.Jayalalitha Fisheries University, Chennai, Tamil Nadu, India
  • S. Gangatharan State Referral Laboratory for Aquatic Animal Health, Tamil Nadu Dr.J.Jayalalitha Fisheries University, Chennai, Tamil Nadu, India
  • N. Palanivel Department of Pathology, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu, India

https://doi.org/10.62757/IVA.2024.101.1.11-14

Keywords:

Tilapia, zoonosis, Brucella melitensis, 16S rRNA gene sequencing

Abstract

Brucellosis is a zoonotic bacterial disease affecting livestock with fewer reports in fishes. The present study reports the isolation and identification of Brucella melitensis from diseased Nile Tilapia, Oreochromis niloticus from a commercial fish farm. The diseased fish with clinical sings were collected and the organs viz., liver, kidney, spleen and intestine were aspectically dissected for isolation of  bacterial pathogens. The bacteria isolate (H1/SRLAAH/2019) from the liver of the infected fish was characterised by the biochemical tests as Brucella sp. and confirmed by 16S rRNA gene sequencing analysis as B.melitensis. This is the first report on the isolation and identification of B. melitensis from O. niloticus in India.

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Submitted

2024-01-19

Published

2024-01-19 — Updated on 2025-12-28

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How to Cite

A. Uma, G. Rebecca, S. Gangatharan, & N. Palanivel. (2025). Isolation and Identification of Brucella Melitensis in Diseased Nile Tilapia, Oreochromis Niloticus from a Commercial Fish Farm in Tamil Nadu, India. The Indian Veterinary Journal, 101(01), 11-14. https://doi.org/10.62757/IVA.2024.101.1.11-14 (Original work published 2024)