Efficacy of Vitamin C and E on the Level of Antioxidative Stress and Steroid Hormones in Albino Rats with Environmental Stress
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Authors
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Winda Kusuma Dewi
Department of Veterinary Science, School of Health and Life Sciences, Universitas Airlangga, Surabaya, 60115, Indonesia
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Faisal Fikri
Department of Veterinary Science, School of Health and Life Sciences, Universitas Airlangga, Surabaya, 60115, Indonesia
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Agus Purnomo
Department of Veterinary Surgery and Radiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia
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Shekhar Chhetri
Department of Animal Science, College of Natural Resources, Royal University of Bhutan, Lobesa, Punakha, 13001, Bhutan
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Salipudin Tasil Maslamama
Department of Agricultural Biotechnology, Faculty of Agriculture, Eskişehir Osmangazi Üniversitesi, Eskisehir, 26160, Turkey
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Muhammad Thohawi Elziyad Purnama
Department of Veterinary Science, School of Health and Life Sciences, Universitas Airlangga, Surabaya, 60115, Indonesia
Keywords:
environmental stress, oxidative stress, steroid hormone, vitamin C, vitamin EAbstract
The aim of this study was to evaluate the efficacy of vitamin C and E in albino rats exposed with environmental stress on the following variables i.e. superoxide dismutase, malondialdehyde, catalase, testosterone, and cortisol level. A total of 20 albino rats were assigned into four treatment groups i.e. (C) normal saline, (T1) vitamin C and E, (T2) induced with stress, (T3) induced with stress and vitamin C and E. All groups were maintained for 4 weeks, respectively. Serum samples were collected from 5 ml of blood, respectively then evaluated using enzymol method for level of antioxidative stress and ELISA method for steroid hormone determination.In result, T3group improved significantly difference compared to T2 group in the variable of malondialdehyde, catalase, testosterone, and cortisol. Meanwhile, there was no significant difference in the superoxide dismutase level between T3 and T2groups. In conclusion, vitamin C and E had an efficacy to ameliorate albino rats condition exposed with environmental stress.
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References
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