Effect of Hydrolysed and Unhydrolysed Whey Protein Bound Iron Supplement on Wistar Rats
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Authors
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Smitha J Lukose
Department of Dairy Chemistry,VKIDFT, KVASU, Mannuthy
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Divya M.P.
Department of Dairy Chemistry, VKIDFT, KVASU, Mannuthy
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Dipu M.T.
Department of Animal Nutrition, CoVAS, KVASU, Mannuthy
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Bibu John Kariyil
Department of Veterinary Pharmacology and Toxicology, CoVAS, KVASU,Mannuthy
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A.K.Beena
Department of Dairy Microbiology, VKIDFT, KVASU, Mannuthy
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S.N. Rajakumar
Department of Dairy Technology, VKIDFT, KVASU, Mannuthy
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Deepa Ananth
Department of Animal Nutrition, CoVAS, KVASU, Mannuthy
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P. Sudheer Babu
8Department of Dairy Engineering, VKIDFT, KVASU, Mannuthy
Keywords:
Whey protein concentrate (WPC), Haemoglobin regeneration efficiency (HRE), Relative biological value (RBV), HydrolysisAbstract
The study evaluated the body stores of iron in anaemic wistar rats after feeding with iron-fortified, whey protein concentrate (WPC)-based supplements. Both hydrolysed and unhydrolysed WPC were used for preparing WPC-iron complexes. Thirty-six male Wistar rats of three weeks of age, divided into six groups, were fed on standard diet, iron-deficient diet, diet with added mineral form of iron, diet with iron as unhydrolysed protein-iron complex ,diet with hydrolysed protein-iron complex , and diet with hydrolysed protein-iron complex with added Vitamin C respectively. Wistar rats that were made anaemic initially by feeding iron-deficient diet were fed the experimental diets with iron supplements later as a part of study. The study indicated that all the three whey based supplements had better iron regeneration capacity than the positive control. The maximum effect was indicated in hydrolysed protein iron complex with Hb of 14.57 g/dL and 48% increase on replenishing. The average Hb of control group was 13.1 g/dL. Vitamin C was not found to have any additional effect on iron bioavailability than that for hydrolysed protein –iron complex.
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References
Allen, L., De Benoist, B., Dary, O. and Hurrell, R. (2006). Guidelines on food fortification with micronutrients. WHO/FAO.
Caetano-Silva, M. E., Bertoldo-Pacheco, M. T., Paes-Leme, A. F. and Netto, F. M. (2015). Iron-binding peptides from whey protein hydrolysates: Evaluation, isolation and sequencing by LC–MS/MS. Food Res. Int , 71, 132-139.
Cappellini, M. D. and Motta, I. (2015). Anemia in clinical practice—Definition and Classification: Does hemoglobin change with aging?. Seminars in hematology, WB Saunders,52(4): 261-269.
Gombart, A. F., Pierre, A. and Maggini, S. 2020. A review of micronutrients and the immune system–working in harmony to reduce the risk of infection. Nutrients, 12(1): 236.
Hurrell, R. F., Lynch, S. R., Trinidad, T. P., Dassenko, S. A. and Cook, J. D. (1989). Iron absorption in humans as influenced by bovine milk proteins. Am. J. Clin. Nutr., 49(3): 546-552.
Jackson, L. S. and Lee, K. (1991). Microencapsulated iron for food fortification. J. Food Sci., 56(4), 1047-1050.
Jacob Filho, W., Lima, C. C., Paunksnis, M. R. R., Silva, A. A., Perilhão, M. S., Caldeira, M.and de Souza, R. R. (2018). Reference database of hematological parameters for growing and aging rats. The Aging Male, 21(2): 145-148.
Kiani, A., Fathi, M. and Ghasemi, S. M. (2017). Production of novel vitamin D3 loaded lipid nanocapsules for milk fortification. Int. J. Food Prop., 20(11): 2466-2476.
Largueza, C. B. D., Mocellin, M. C., Nunes, J. C. and Ribas, S. A. (2023). Effect of intake of iron-fortified milk on levels of ferritin and hemoglobin in preschoolers: A systematic review and meta-analysis. Clinical Nutrition ESPEN.
Lukose, S.J, Murthy N and jum A (2018) Effect of hydrolysis on allergenicity and sensory quality of whey protein concentrate.Int. J. Curr. Microbiol. Appl. Sci.,7(05): 2899–2905.
Lobo, A.R., Cocato, M.L.,Borelli, P., Gaievski, E. H. S., Crisma, A.R., Nakajima, K., Nakano, E. Y.and Colli, C. (2011).Iron bioavailability from ferric pyrophosphate in rats fed with fructan-containing yacon (Smallanthus sonchifolins) flour. Food Chem.126:885-891.
Mannar, V. and Gallego, E. B. 2002. Iron fortification: country level experiences and lessons learned. J Nutr., 132(4), 856S-858S.
Miano, A. C. and Rojas, M. L. (2023). Engineering strategies for food fortification. Current Opinion in Food Science, 101033.
Nakano, T., Goto, T., Nakaji, T. and Aoki, T. (2007). Bioavailability of iron-fortified whey protein concentrate in iron-deficient rats. Asian-australas. J. Anim. Sci., 20(7): 1120-1126.
Piskin, E., Cianciosi, D., Gulec, S., Tomas, M. and Capanoglu, E. (2022). Iron absorption: factors, limitations, and improvement methods. ACS omega, 7(24): 20441-20456.
Sabatier, M., Rytz, A., Husny, J., Dubascoux, S., Nicolas, M., Dave, A. and Glahn, R. P. (2020). Impact of ascorbic acid on the in vitro iron bioavailability of a casein-based iron fortificant. Nutrients, 12(9): 2776.
Savarino, G., Corsello, A. and Corsello, G. (2021). Macronutrient balance and micronutrient amounts through growth and development. Ital. J. Pediatr., 47(1): 1-14.
Shenkin, A. 2006. Micronutrients in health and disease. Postgrad. Med. J., 82(971): 559-567.
Shubham, K., Anukiruthika, T., Dutta, S., Kashyap, A. V., Moses, J. A. and Anandharamakrishnan, C. (2020). Iron deficiency Anemia: A comprehensive review on iron absorption, bioavailability, and emerging food fortification approaches. Trends Food Sci Technol., 99: 58-75.
Stekel, A., Olivares, M., Pizarro, F., Chadud, P., Lopez, I., and Amar, M. 1986. Absorption of fortification iron from milk formulas in infants. Am. J. Clin. Nutr., 43(6), 917-922.
Susanti, T., Dirgahayu, P.and Indarto, D.(2017). Development of rat model with iron deficiency anemia by modification of its standard food. Adv. Health Sci. Res. 9:63-66.
Turawa, E., Awotiwon, O., Dhansay, M. A., Cois, A., Labadarios, D., Bradshaw, D. and Pillay-van Wyk, V. (2021). Prevalence of anaemia, iron deficiency, and iron deficiency anaemia in women of reproductive age and children under 5 years of age in south africa (1997–2021): A Systematic Review. Int. J. Environ. Res. Public Health, 18(23): 12799.
Venkatasubramanian, P., Koul , I.B.,Varghese, R.K., Srividya, K. and Arun,S. (2014).Amla (Phyllanthus emblica) enhances iron dialysability and uptake in vitro models. Curr. Sci. 107(11):1859-1866.
Villalpando, S., Shamah, T., Rivera, J. A., Lara, Y. and Monterrubio, E. (2006). Fortifying milk with ferrous gluconate and zinc oxide in a public nutrition program reduced the prevalence of anemia in toddlers. J. Nutr., 136(10): 2633-2637.
Walczyk, T., Muthayya, S., Wegmüller, R., Thankachan, P., Sierksma, A., Frenken, L. G. and Hurrell, R. F. (2014). Inhibition of iron absorption by calcium is modest in an iron-fortified, casein-and whey-based drink in Indian children and is easily compensated for by addition of ascorbic acid. J. Nutr, 144(11), 1703-1709.
Wu, H., Liu, Z., Zhao, Y. and Zeng, M. (2012). Enzymatic preparation and characterization of iron-chelating peptides from anchovy (Engraulis japonicus) muscle protein. Food Res. Int., 48(2), 435-441.
Zhang, D., Hendricks, D. G. and Mahoney, A. W. (1989). Bioavailability of total iron from meat, spinach (Spinacea oleracea L.) and meat–spinach mixtures by anaemic and non-anaemic rats. Br. J. Nutr., 61(2), 331-343.
