Comparison of Bacterial Contamination in Sea Turtle Natural and Semi-natural Hatching Nest at Boom Beach Banyuwangi


334 / 43

Authors

  • Ratih Novita Praja Veterinary Medicine Study Program, Department of Health and Life Sciences, School of Health and Life Sciences,Universitas Airlangga, Wijaya Kusuma Street 113, Banyuwangi, East Java, Indonesia
  • Aditya Yudhana Veterinary Medicine Study Program, Department of Health and Life Sciences, School of Health and Life Sciences,Universitas Airlangga, Wijaya Kusuma Street 113, Banyuwangi, East Java, Indonesia
  • Wiyanto Haditanojo Banyuwangi Sea Turtle Foundation; Wahid Hasyim Street 42, Banyuwangi, East Java, Indonesia
  • Didik Handijatno Veterinary Medicine Study Program, Department of Health and Life Sciences, School of Health and Life Sciences,Universitas Airlangga, Wijaya Kusuma Street 113, Banyuwangi, East Java, Indonesia
  • Muhammad Thohawi Elziyad Purnama Veterinary Medicine Study Program, Department of Health and Life Sciences, School of Health and Life Sciences,Universitas Airlangga, Wijaya Kusuma Street 113, Banyuwangi, East Java, Indonesia
  • Salipudin Tasil Maslamama Department Agricultural Biotechnology, Eskişehir Osmangazi Üniversitesi, Ali NumanKıraç Campus, 26160 Eskişehir, Turkey
  • Alivia Khairina Insani Student of Veterinary Medicine Study Program, School of Health and Life Sciences, Universitas Airlangga, Wijaya Kusuma Street 113, Banyuwangi, East Java, Indonesia
  • Dhenatra Rifqy Prasetyo Student of Veterinary Medicine Study Program, School of Health and Life Sciences, Universitas Airlangga, Wijaya Kusuma Street 113, Banyuwangi, East Java, Indonesia
  • Nindya Pradnya Prameswari Student of Veterinary Medicine Study Program, School of Health and Life Sciences, Universitas Airlangga, Wijaya Kusuma Street 113, Banyuwangi, East Java, Indonesia
  • Shifa Salsabilla Praja Student of Veterinary Medicine Study Program, School of Health and Life Sciences, Universitas Airlangga, Wijaya Kusuma Street 113, Banyuwangi, East Java, Indonesia

https://doi.org/10.62757/IVA.2024.101.4.7-11

Keywords:

Infectious disease, Marine biodiversity, Sea turtle, Total Plate Count

Abstract

This study aims to investigate the total number of bacterial contamination in sea turtle natural hatching nest and semi-natural hatching nest. Sand samples were taken from sea turtle natural and semi-natural hatching nest located where at Boom beach, Banyuwangi, East Java Province, Indonesia. Total Plate Count (TPC) method was used in this study. The results show a total of 2,9 x 106 colonies were detected from natural hatching nest, while a total of 2,3 x 106 bacterial colonies were detected from semi-natural hatching nest. This research concluded that bacterial colonies were obtained from natural and semi-natural hatching nests. Furthermore, further studies need to be carried out regarding the isolation of specific bacteria using culture media to determine the species of pathogenic bacteria that have the potential to reduce the success of turtle egg hatching.

Downloads

Download data is not yet available.

References

Al-Bahry, S., Mahmoud, I., Melghit, Y., and Al-Amri, K. (2011). Analysis of Elemental Composition of the Eggshell Before and After Incubation in the Loggerhead Turtle Caretta caretta in Oman. Microsc. Microanal. (17): 1-9.

Bezy, V.S., Valverde, R.A., and Plante, C.J. (2015). Olive Ridley Sea Turtle Hatching Success as a Function of The Microbial Abundance in Nest Sand at Ostional, Costa Rica. PLoS One 10 (2): 1-24.

Booth, D.T. and Dunstan, A.(2018). A preliminary investigation into the early embryo death syndrome (EEDS) at the world’s largest green turtle rookery. PloS one, 13(4), p.e0195462.

Cafarchia, C., Paradies, R., Figueredo, L. A., Iatta, R., Desantis, S., Di Bello, A. V. F., Zizzo, N., and van Diepeningen, A. D. (2020) Fusarium spp. in Loggerhead Sea Turtles (Caretta caretta): From Colonization to Infection. Vet. Pathol. 57(1): 139-146.

Candan, A.Y., Katılmış, Y., and Ergin, Ç. (2021). First Report of Fusarium Species Occurrence in Loggerhead Sea Turtle (Caretta caretta) Nests and Hatchling Success in Iztuzu Beach, Turkey. Biologia, 76 : 565-573.

Candan, O., and Candan, E.D. (2020). Bacterial Diversity of Green Turtle (Chelonia mydas) Nest Environment. Sci. Total Environ 720: 1-10.

Caron, A.G.M, Thomas, C.R., Berry, K.L.E, Motti, C.A., Ariel, E., and Brodie, J.E. (2018). Ingestion of Microplastic Debris by Green Sea Turtle (Chelonia mydas) in the Great Barrier Reef: validation of a sequential extraction protocol. Mar. Pollut. Bull. 127: 743-751.

Convention on International Tradein Endangered Species of Wild Flora and Fauna (CITES). (2015). Checklist of CITES Species. Accessed 15 September 2021 3.23AM in http://checklist.cites.org.

De Andrés, E., Gómara, B., González-Paredes, D., Ruiz-Martín, J., and Marco, A. (2016). Persistent Organic Pollutant Levels in Eggs of Leatherback Sea Turtle (Dermochelys coriacea) Point to a Decrease in Hatching Success. Chemosphere 146: 354-361.

Estika, A. (2013). Analisis Mikroorganisme Pada Telur Penyu Hijau (Chelonia Mydas) Dari Pulau Baling – Balingan, Kalimantan Timur. Prosiding Seminar Nasional. Biologi. UPI. Bandung.

Gifari T., Elfidasari D., and Sugoro I. (2018). The Effects of Contaminant Microorganism TowardsChelonia mydas Eggs Hatchery Result in Pangumbahan Green Sea Sea turtle Conservation, Sukabumi, Indonesia. Biodiversitas 19 (4): 1207-1212.

Gleason, F. H., Allerstorfer, M., and Lilje, O. (2020). Newly emerging diseases of marine turtles, especially sea turtle egg fusariosis (SEFT), caused by species in the Fusarium solani complex (FSSC). Mycology, 11(3), 184-194.

Harahap, I. Minarti., A. Fahrudin., and Y. Wardiatno. (2015) Pengelolaan Kolaboratif Kawasan Konservasi Penyu Pangumbahan Kabupaten Sukabumi. Jurnal Ilmu Pertanian Indonesia (JIPI) 20 (1): 39 46.

Hoh D.Z., Lin Y.F., Liu W.A., Sidique S.N.M., and Tsai I.J. (2019). Nest Microbiota and Pathogen Abundance Impact Hatching Success in Sea Turtle Conservation. bioRxiv.

International Union for Conservation of Nature (IUCN). (2008). The IUCN Red List of Threatened Species. ISSN 2307 -8235.

Praja, R.N., Yudhana, A., Haditanojo, W., Kusumawardani, A., Putri, A.N.J., Fadillah, I.R., Pratama, L.D., and Afiyah, N.S. (2023). A Short Note on Fusarium spp Identification from Eggs of Olive Ridley Sea Turtle (Lepidochelys olivacea) in Banyuwangi, East Java, Indonesia. Indian Vet. J., 100(5) : 19-22.

Praja, R. N., Yudhana, A., Haditanojo, W., and Oktaviana, V. (2021). Antimicrobial Properties in Cloacal Fluid of Olive Ridley Sea Turtle (Lepidochelys olivacea). Biodiversitas, 22(9).

Phillott, A.D., and Parmenter, C.J. (2001). The Distribution of Failed Eggs and the Appearance of Fungi in Artificial Nests of Green (Chelonia mydas) and Loggerhead (Caretta caretta) Sea Turtle. Aust. J. Zool., 49(6) : 713-718.

Samosir, S.H., Hernawati, T., Yudhana, A. and Haditanojo, W., (2018) Perbedaan sarang alami dengan semi alami mempengaruhi masa inkubasi dan keberhasilan menetas telur penyu lekang (Lepidochelys olivacea) pantai boom Banyuwangi. JMV, 1(2) : 33-37.

Soslau G., Spotila J.R., Chun A., Yi S., and Weber K.T. (2011). Potentially Lethal Bacteria in Leatherback Turtle Eggs in the Wild Threaten Both Sea Turtle and Conservationists. FEMS Microbiol Lett 410: 101-106.

Wicaksono, M. A., Nurhasanah, F., Elfidasari, D., and Sugoro, I. (2018). Cemaran Mikroba pada Telur Penyu Sisik (Eretmochelys Imbricata) di Pulau Kelapa Dua, Taman Nasional Laut Kepulauan Seribu, DKI Jakarta. Jurnal Al-Azhar Seri Sains dan Teknologi. 4(2) : 83 – 90.

Downloads

Submitted

2024-04-25

Published

2024-04-25 — Updated on 2025-12-27

Versions

How to Cite

Ratih Novita Praja, Aditya Yudhana, Wiyanto Haditanojo, Didik Handijatno, Muhammad Thohawi Elziyad Purnama, Salipudin Tasil Maslamama, Alivia Khairina Insani, Dhenatra Rifqy Prasetyo, Nindya Pradnya Prameswari, & Shifa Salsabilla Praja. (2025). Comparison of Bacterial Contamination in Sea Turtle Natural and Semi-natural Hatching Nest at Boom Beach Banyuwangi. The Indian Veterinary Journal, 101(4), 7-11. https://doi.org/10.62757/IVA.2024.101.4.7-11 (Original work published 2024)