In vitro Evaluation of Andrographolide Stability in Rumen Fluid to explore oral Administration of Andrographis paniculata in Ruminants
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Authors
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M.R. Srinivasan
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K.G. Thirumurugaan
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S.Rithik
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P. Pandikumar
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S. Ignasimuthu
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S. Ramesh
Keywords:
Ruminants, Andrographolide, AndrographispaniculataAbstract
Andrographis paniculata (Burm. f.) Wall. Ex Nees is an important medicinal plant used in both human and veterinary healthcare, and andrographolide is the major active constituent of A. paniculata. This study communicates the stability of andrographolide in the rumen fluid when administered in different forms. The stability of andrographolide was assessed using an in vitro method, in which coarsely powdered aerial parts of A. Paniculata (10 mg/mL), a standardized extract of A. paniculata (200 μg/mL), and pure andrographolide (5 μg/mL) were separately incubated with rumen fluid, and the level of andrographolide in the rumen fluid was estimated at 1, 2, 4, 8, and 24 h after incubation. This study showed that approximately 90.9% of andrographolide vanished rapidly from the rumen fluid when it was added to the rumen fluid in its pure form, whereas the concentration of andrographolide progressively increased by 167.91 and 200% when the hydroalcoholic extract of A. paniculata or dried aerial part of the plant was incubated with rumen fluid, respectively. This study provides confidence in administering the A. paniculata extract or the dried aerial parts orally in ruminants; however, it warrants further detailed investigation on the pharmacokinetics of andrographolide in ruminants upon oral administration of the A. paniculata plant or extract.
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