Molecular Study of Toll-Like Receptor 2 Gene of Punganur cattle
24 / 11
Authors
-
Sai Teja
-
R.K. Chaitanya
-
K. Lakshmi Kavitha
-
G. Shushma
Keywords:
Punganur, TLR2, PCR, characterisationAbstract
Toll-like receptors are type 1 transmembrane proteins expressed in almost all cell types and activate the innate immune system. The present study involved characterization of TLR2 gene of Punganur cattle, a dwarf indigenous cattle breed native to Chittoor district of Andhra Pradesh. The exon 2 of TLR2 gene that contains the entire coding sequence of 2355 bp (784 amino acids) was targeted in two PCR reactions and amplified into two overlapping fragments. Both the specific amplicons are sequenced by Sanger’s method. The nucleotide sequence analysis of TLR 2 gene of Punganur cattle revealed 99.38 per cent identity with the TLR2 sequence of Vechur cattle of Kerala (KT862891; Bos indicus) and 99.9-100 per cent homology with Bos taurus TLR2 sequences (AY 634629, XM 027513488.1) except for the two non-synonymous mutations resulting in animo acid variations Asp63Glu, Ile211Val.
Downloads
References
Akira, S., S.Uematsu and O.Takeuchi (2006), Pathogen recognition and innate immunity, Cell, 124(4): 783-801. Buwitt-Beckmann, U., H.Heine, K.H.Wiesmüller, G.Jung, R.Brock, S.Akira and A.J.Ulmer (2006), TLR1-and TLR6-independent recognition of bacterial lipopeptides, Journal of Biological Chemistry, 281(14): 9049-9057. Chang, J. S., G.C.Russell, O.Jann, E.J.Glass, D.Werling and D.M.Haig (2009), Molecular cloning and characterization of Toll-like receptors 1-10 in sheep, Veterinary immunology and immunopathology, 127(1-2): 94-105. Ma, X., Y.Liu, B.B.Gowen, E.A.Graviss, A.G.Clark and J.M.Musser (2007), Full-exon resequencing reveals toll-like receptor variants contribute to human susceptibility to tuberculosis disease, PloS one, 2(12): e1318. Mariotti, M., J.L.Williams, S.Dunner, A.Valentini and L.Pariset (2009), Polymorphisms within the toll-like receptor (TLR)-2,-4, and-6 genes in cattle, Diversity, 1(1): 7-18. Seabury, C.M. and J.E.Womack (2008), Analysis of sequence variability and protein domain architectures for bovine peptidoglycan recognition protein 1 and Toll-like receptors 2 and 6, Genomics, 92(4): 235-245. Subhash, V., S.Monika, S.Richa, S.Chander, S.Geetanjali and S.Mandeep (2018), Distribution of single-nucleotide polymorphisms and protein domain architecture of toll-like receptor-2 in Pahari cattle (Indian non-descript indigenous breed), Research in Veterinary Science, 117, 144-149.
Downloads
Submitted
Published
Versions
- 2026-03-24 (2)
- 2026-01-29 (1)