Elucidation of the Heparin Binding Domain of Goat Vitronectin by Deletion Mutagenesis
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Authors
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Murugavel Sokalingam
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Paritosh Joshi
Keywords:
Goat vitronectin, Heparin binding domain, HBD, Deletion mutagenesisAbstract
Vitronectin is a multifunctional glycoprotein present in the extracellular matrix. The RGD motif and the heparin-binding domains of the human protein play a major role in cell adhesion and bacterial binding. Goat vitronectin is unique in having two RGD motifs compared to human vitronectin. The heparin-binding domain of goat vitronectin has not yet been identified, and the amino acids from 340 to 380 of human vitronectin are known to be involved in heparin binding primarily. The proposed HBD of goat protein contains consensus sequences of many known heparin-binding proteins. PCR-based deletion mutagenesis of this region indicated that the basic residues are essential for heparin binding and protein multimerisation.
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References
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