Multiple drug resistant Edwardsiella tarda associated disease outbreak in Labeo catla
14 / 12
Authors
-
Dr A. Uma
Tamil Nadu Dr.J.Jayalalithaa Fisheries University, Nagapattinam
-
Dr S.Ganesh Babu
Tamil Nadu Dr J.Jayalalithaa Fisheries University, Nagapattinam
-
Miss G Rebecca
Tamil Nadu Dr J.jayalalithaa Fisheries university, Nagapattinam
Keywords:
Edwardsiella tarda, fimbrial gene, antibiotic resistanceAbstract
The present study documented an Edwardsiella tarda associated disease outbreak in a Labeo catla commercial fish farm in Tamil Nadu, India. Samples of infected fish (n=10) were collected from the L.catla farm in Thanjavur, Tamil Nadu, India that had reported an outbreak of disease. Bacterial isolation was done from kidney, eye, liver, intestine, brain and gills and the isolates were biochemically characterized, confirmed by PCR amplification and nucleotide sequencing as E.tarda (E4L/SRLAAH/2020). Antibiogram showed that the isolate was either resistant or intermediately resistant to Chloramphenicol, Ampicillin, Oxytetracycline, Cefotaxime, Vancomycin, Amoxicllin, Erythromycin, Ceftazidime, Kanamycin and Tetracycline and sensitive to five antibiotics viz., Norfloxacin, Ciprofloxacin, Nalidixic acid, Oflaxacin and Co-trimazole.
Downloads
References
Alanis, A.J., 2005. Resistance to antibiotics: are we in the post-antibiotic era? Arc.med. res., 36(6):697-705.
Algammal, A.M., Mabrok, M., Ezzat, M., Alfifi, K.J., Esawy, A.M., Elmasry, N. and El-Tarabili, R.M., 2022. Prevalence, antimicrobial resistance (AMR) pattern, virulence determinant and AMR genes of emerging multi-drug resistant Edwardsiellatarda in Nile tilapia and African catfish. Aqua., 548:737643.
Bauer, A.W., Kirby, W.M.M., Sherris, J.C. and Turck, M., 1966. Antibiotic susceptibility testing by a standardized single disk method. Ame. J. of clini. Pathol., 45(4):493-496.
Choudhury, T.G., Vinay, T.N., Gupta, S.K., Gita, S. and Sarkar, B., 2017. Edwardsiellosis: An emerging disease in Indian aquaculture. Aquacul. Times, 3(2);38-40.
Grimont, P.A., Grimont, F., Richard, C. and Sakazaki, R., 1980. Edwardsiellahoshinae, a new species of Enterobacteriaceae. Curr. Microbiol., 4(6):347-351.
Leung, K.Y., Siame, B.A., Tenkink, B.J., Noort, R.J. and Mok, Y.K., 2012. Edwardsiellatarda–virulence mechanisms of an emerging gastroenteritis pathogen. Micro and infec., 14(1):26-34.
Ling, S.H., Wang, X.H., Lim, T.M. and Leung, K.Y., 2001. Green fluorescent protein-tagged Edwardsiellatarda reveals portal of entry in fish. FEMS microbial. lr, 194(2):239-243.
Mahmoud, M.M., 2006. Induction of hemagglutinating activity of Edwardsiellatarda by sodium chloride., 41(1):29-34.
Meyer, F.P. and Bullock, G.L., 1973. Edwardsiellatarda, a new pathogen of channel catfish (Ictalurus punctatus). App. microbiol, 25(1):155-156.
Miniero Davies, Y., Xavier de Oliveira, M.G., Paulo Vieira Cunha, M., Soares Franco, L., Pulecio Santos, S.L., Zanolli Moreno, L., Túlio de Moura Gomes, V., Zanolli Sato, M.I., Schiavo Nardi, M., Micke Moreno, A. and Becker Saidenberg, A., 2018. Edwardsiellatarda outbreak affecting fishes and aquatic birds in Brazil. Vet. Quar, 38(1):99-105.
Mohanty, B.R. and Sahoo, P.K., 2007. Edwardsiellosis in fish: a brief review. J. biosci, 32(3):1331-1344.
Muratori, M.C.S., De Oliveira, A.L., Ribeiro, L.P., Leite, R.C., Costa, A.P.R. and Da Silva, M.C.C., 2000. Edwardsiellatarda isolated in integrated fish farming. Aquac. Res., 31(6):481-483.
Noga, E.J., 2010. Fish disease: diagnosis and treatment. John Wiley & Sons.
Ogunleye, S.C., Ishola, O.O., Olatoye, I.O. and Adedeji, O.B., 2020. Detection and antibiogram of Edwardsiellatarda from Oreochromis niloticus (Tilapia) obtained from selected farms in Ibadan, Nigeria. J. Food Saf. . Hy., 6(1):38-46
Park, S.B., Aoki, T. and Jung, T.S., 2012. Pathogenesis of and strategies for preventing Edwardsiellatardainfection in fish. Vet.res., 43(1): 1-11.
Rahman, M., Rahman, M.M., Deb, S.C., Alam, M.S., Alam, M.J. and Islam, M.T., 2017. Molecular identification of multiple antibiotic resistant fish pathogenic Enterococcus faecalis and their control by medicinal herbs. Sci. rep., 7:3747.
Reger, P.J., Mockler, D.F. and Miller, M.A., 1993. Comparison of antimicrobial susceptibility, β-lactamase production, plasmid analysis and serum bactericidal activity in Edwardsiella tarda, E. ictaluri and E. hoshinae. J. Med. Microbiol., 39(4):273-281.
Sakai, T., Iida, T., Osatomi, K. and Kanai, K., 2007. Detection of type 1 fimbrial genes in fish pathogenic and non-pathogenic Edwardsiella tarda strains by PCR. Fis. Pathol, 42(2):115-117.
Shotts, E.B. and Waltman, W.D., 1990. A medium for the selective isolation of Edwardsiella ictaluri J. Wildlife Dis., 26(2):214-218.
Srinivasa Rao, P.S., Lim, T.M. and Leung, K.Y., 2003. Functional genomics approach to the identification of virulence genes involved in Edwardsiella tarda pathogenesis. Inf. Immu., 71(3):1343-1351.
Stock, I. and Wiedemann, B., 2001. Natural antibiotic susceptibilities of Edwardsiellatarda, E. ictaluri, and E. hoshinae. Anti. agents and chemo., 45(8):2245-2255.
Szekely, C., Cech, G., Chaudhary, A., Borzák, R., Singh, H.S. and Molnár, K., 2015. Myxozoan infections of the three Indian major carps in fish ponds around Meerut, UP, India, with descriptions of three new species, Myxobolus basuhaldari sp. n., M. kalavatiae sp. n. and M. meerutensis sp. n., and the redescription of M. catlae and M. bhadrensis. Parasitology research, 114(4), pp.1301-1311.
Taoka, Y., Maeda, H., Jo, J.Y., Kim, S.M., Park, S.I., Yoshikawa, T. and Sakata, T., 2006. Use of live and dead probiotic cells in tilapia Oreochromis niloticus. Fish. Sci., 72(4): 755-766.
Thune, R.L., Stanley, L.A. and Cooper, R.K., 1993. Pathogenesis of gram-negative bacterial infections in warmwater fish. Ann. Rev. .Fish Dis., 3:37-68.
Uma, A., Jawahar Raj, K., Rebecca, G. and Anand, S., 2014, Antibiotic Resistance in Aeromonas Hydrophila Isolated from Clinically Infected Cat fish, Clarius sp.Indian Vet. J., 91: 55 – 58
Uma, A., Stalin, N. and Rebecca, G.P., 2017. Isolation, molecular identification and antibiotic resistance of Enterococcus faecalis from diseased tilapia. Int. J. Curr. Microbiol. Appl. Sci, 6:136-146.
Uma, A., Harresh, A.H.M., Rebecca, G. and Praveenraj, J., 2020. Multiple drug resistant Enterococcus spp. causes disease and mortality in Zebra fish (Danio rerio). The Ind. J. Ani. Sci.90:116-119.
Vishnupriya, V., Swaminathan, T.R., Dharmarathnam, A., Sharma, S.R. and Preena, P.G., 2024. Virulent and Multi-drug-Resistant Edwardsiella tarda Infection in Oscar Fish: Unveiling the Threat of Mass Mortality and AMR Dissemination. Cur. Microbiol., 81:1-16.
Wimalasena, S.H.M.P., Pathirana, H.N.K.S., De Silva, B.C.J., Hossain, S., Sugaya, E., Nakai, T. and Heo, G.J., 2018. Antibiotic resistance and virulence-associated gene profiles of Edwardsiellatarda isolated from cultured fish in Japan. Tur. J. Fis. Aqu. Sci., 19(2):141-147.
Wyatt, L.E., Nickelson 2nd, R. and Vanderzant, C.A.R.L., 1979. Edwardsiella tarda in freshwater catfish and their environment. App. Env. Microbiol., 38(4):710-714.
