EFFECT OF DIETARY SUPPLEMENTATION OF MANNANOLIGOSACCHARIDE AND BACILLUS SUBTILIS ON GROWTH PERFORMANCE, IMMUNITY, DISEASE RESISTANCE AND DIGESTIVE ENZYME ACTIVITIES OF LABEO FIMBRIATUS FINGERLINGS
52
Authors
-
N. A. Pawar
Central Marine Fisheries Research Institute, Mumbai Regional Centre, Mumbai-400061, India
-
Chandra Prakash
Aquaculture Division, Central Institute of Fisheries Education, Mumbai-400061, India
-
M.P.S. Kohli
Aquaculture Division, Central Institute of Fisheries Education, Mumbai-400061, India
-
S. K. Singh
College of Fisheries, Central Agricultural University, Lembucherra-799210, India
-
Neelam Saharan
Aquaculture Division, Central Institute of Fisheries Education, Mumbai-400061, India
-
S. B. Jadhao
Aquaculture Division, Central Institute of Fisheries Education, Mumbai-400061, India
-
B. N. Devi
College of Fisheries, Chhattisgarh Kamdhenu University, Raipur-491995, India
-
A. K. Chaudhari
Aquaculture Division, Central Institute of Fisheries Education, Mumbai-400061, India
Abstract
A study was conducted for 60 day period to delineate the individual and combined effect of dietary mannanoligosaccharide (MOS) and Bacillus subtilis (BS) supplementation on growth and immune status of Labeo fimbriatus fingerlings. Results illustrated that the inclusion of MOS alone did not significantly (P>0.05) improve the overall growth compared to TC. Groups fed 104 CFU g-1 B.subtilis had positive effect on % weight gain, SGR, FER and FCR. The 0.5% MOS supplementation along with 104 CFU g-1 B. subtilis (T3) had exhibited significantly higher growth performance, highest total serum protein or globulin value in L. fimbriatus fingerlings, suggesting the optimum combination level. Growth retardation and immune-suppression at higher doses at 1% MOS and 106 CFU g-1 of  B.subtilis was evident. Further, dietary supplementation of MOS significantly affected blood indices and a significant interaction was occurred due to MOS. Dietary treatment T5 elicited strongest protease and amylase activity and lowest lipase activity (U mg-1 protein) among all the dietary treatments. There was significant effect of MOS (amylase activity), MOS and B.subtilis alone or in combination (P>0.05) on intestinal protease activity of L. fimbriatus fingerlings. The percentage survival in dietary treatments was significantly affected by MOS levels (P>0.05) and there was interaction between MOS and B. subtilis supplemented groups due to MOS levels. The results suggest that adequate incorporation level of these supplements is crucial for improving growth as well as immunity of l. fimbriatus fingerlings.
Keywords: Combined effect, mannanoligosaccharid, immune status, globulin, immune suppression, Labeo fimbriatus.
