GENETIC ANAlYSIS OF EAST AND WEST COAST POPUlATIONS OF PENAEUS MONODOIV INDIA BASED ON RANDOM AMPliFIED POlYMORPHIC DNA
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Authors
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Prem Sagar Mishra
Division of Fish Genetics and Biotechnology_ Central Institute of Fisheries Education
Panch Marg, Versova, Mumbai- 400 061, India.
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W S Lakra
Division of Fish Genetics and Biotechnology_ Central Institute of Fisheries Education
Panch Marg, Versova, Mumbai- 400 061, India..
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Aparna Chaudhari
Division of Fish Genetics and Biotechnology_ Central Institute of Fisheries Education
Panch Marg, Versova, Mumbai- 400 061, India..
Abstract
The east and west coast populations of wild Penaeus monodon in India were geneticai~y
characterized by RAPD analysis using six highly polymorphic primers reported earlier. The av.erage
genetic similar~ties within populations, based on profiles generated by all the six primers, were
0.828 and 0.851 for the east and west coast populations, respectively, values with individual
primers ranging from 0.744 to 0.889. The average genetic similarity between populations across
ail the primers was 0.774. The number of bands found to be polymorphic were 38 (51.35%}
(50.68%) in the east and west coast populations, respectively. Primer 5 yielded tne highest ieveu
polymorphism (63.63%) in the east coast population whereas primer 3 yielded the lowest level of
polymorphism (36.36%) in the west coast population. The study reveals the existence of genetu•t
variation in P. monodon stocks providing scope for genetic improvement through selectilve
breeding. It also provides baseline data for future work on population structure analysis o'J iP,
monodon.
Key words: RAPD, P. monodon, genetic diversity, polymorphism.
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