ESTIMATION OF MICRO-FlORA ASSOCIATED WITH DIFFERENT STAGES OF MACROBRACHIUM ROSENBERGII (de Man)
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Authors
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Laxmi Prasad
Directorate of Extension, N.D.U.A. & T., Kumarganj_ Faizabad,
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B. B. Nayak
Central Institute of Fisheries Education (Deemed University), Versova, Mumbai, 400061, India
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A. K. Reddy
Central Institute of Fisheries Education (Deemed University), Versova, Mumbai, 400061, India
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P. P. Srivastava
NBFGR (ICAR), Lucknow
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M.P. S. Kohli
Central Institute of Fisheries Education (Deemed University), Versova, Mumbai, 400061, India
Abstract
The bacterial flora occurring in muscle, haemolymph, hepatopancreas and gill of brood, juveniles, water, eggs, larvae and rearing water were estimated by selective plate count technique for Entrobacteriaceae, Streptococaceae, and Vibrionaceae members. The total viable bacterial count was estimated by total plate count technique on nutrient agar. The total viable counts of bacteria were lowest in water from (6.10x102CFU/ml) and highest in egg {6.06x108 CFU/g). In brood the
total counts were varying from 1.62x10 2CFU/g in muscle to 2.20x105 CFU/g in gills. In juveniles,the total plate counts were varying from 2.8x104 CFU/g in muscles to 3.67x108 CFU/g in hepatopancreas. Selective plate counts show that Enterobacteriaceae members dominate in egg and gills of brood and hepatopancreas of juveniles. Vibrios were found to be dominant in water and larvae of rearing tank. Haemolymph of brood was sterile and did not contain any bacteria while muscle of juvenile was having very low count of total viable bacteria.
Keywords- Macrobrachium rosenbergii, egg, larvae, haemolymph, hepatopancreas.
