EFFECT OF NEEMTA 2100 TOXICITY ON ACETYLCHOLINESTERASE AND SERUM GLUTAMATE OXALOACETATE TRANSAMINASE ENzynms IN SERUM OF FISH, OREOCHROMIS MOSSAMBICUS
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Abstract
Acetylcholinesterase and Serum glutamate oxaloacetate transaminase
enzymes have been used as marker monitoring the effect of neem seed based
pesticide Neemta 2100 on the fish, Oreochromis mossambicus. Fishes exposed
to sublethal concentrations of neemta 2100 for acute periods of 24 and 48
hours were sacrificed to determine enzyme activities in serum affected due
to toxicity. Laboratory studies of in vivo exposure of this pesticide showed
synergistic inhibitory effect during acute period of toxicity.
Acetylcholinesterase was noticed as 6.25 J.Uil substrate hydrolyzed I mg protein
I hour and Serum glutamate oxaloacetate transaminase was noticed as 36.71
J.Uil substrate hydrolyzed I mg protein I hour in control fish serum. Significant
decrease in GOT level in Neemta 2100 treated fishes after short term exposure
indicated its severe toxicity to fish.
Key words: Serum, Neemta 2100, Enzymes, Fish